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  • HE Swift Cloning Kit

    HE Swift Cloning Kit

    TOOLS HE Swift Cloning Kit is an advanced positive selection system for the highest efficiency cloning of PCR products generated with Pfu DNA polymerase, Taq DNA polymerase, or other thermo-stable DNA polymerases. Additionally, any other DNA fragment, either blunt or sticky-end, can be successfully cloned using the kit. Cloning is fast and efficient; ligation takes only 5 minutes and yields more than 99% positive clones.
    The kit features the novel positive selection cloning vector pHE. This vector contains a lethal gene which is disrupted by ligation of a DNA insert into the cloning site. As a result, only cells with recombinant plasmids are able to propagate, eliminating the need for expensive blue/white screening.
    The vector contains an expanded multiple cloning site, as well as a T7 promoter for in vitro transcription. Sequencing primers are included for convenient sequencing of the insert.

    Cloning Principle
    Quick and efficient: Blue/white screening is not necessary, and the whole ligation process can be finished within 5 min.
    Sensitive and widely applied: Good for the ligation of low concentration fragments and long fragments, works for both blunt and sticky end product.

  • DNA View

    DNA View

      TOOLS DNA view is an innovated nucleic acid stain to replace highly toxic ethidium bromide (EtBr) for detecting nucleic acid agarose gels. It emits green fluorescence when bound to DNA and RNA. TOOLS DNA view is as sensitive as EtBr and the staining protocol is also very similar, however, compared to EtBr, which is known as a strong mutagen, TOOLS DNA view causes much fewer mutations in the Ames test. In addition, it has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. Most importantly, it is not considered hazardous waste, can be disposed of according to standard laboratories procedures, and is stable for years.

  • Cytoplasmic and Nuclear Protein Extraction Kit

    Cytoplasmic and Nuclear Protein Extraction Kit

      This kit supplies a complete set of lysis reagents that enable the separation of nuclear protein and cytoplasmic fractions from cultured cells and tissue. The optimized reagents and protocol allow non-denatured, active proteins to be purified in less than two hours. It is sufficient for extracting 50-100 protein samples, the quantity for each sample is (5-10) x 108 cells (about 50mg).

     

  • 小鼠基因編輯(CRISPR/Cas9 system)服務

    小鼠基因編輯(CRISPR/Cas9 system)服務

    圖爾思生物科技公司為台灣最早開始推展CRISPR/Cas9技術且為最先開始利用此系統提供客製化的基因編輯小鼠與大鼠。我們利用in vitro transcripted sgRNA與獨有的Cas9 protein,在單一個受精卵時期用microinjection方式將材料打入,六至八週九可以產出F0小鼠。

  • 蛋白質定量(i-TRAQ)

    蛋白質定量(i-TRAQ)

    i-TRAQ的全名為Isobaric tags for relative and absolute quantitation ,亦即利用同位素”標籤”將來自不同樣本的蛋白用不同的同位素標記,造成在二級質譜圖分析時不同樣本來源的蛋白會有不同的分子量,因此可以區分出不同樣本來源的蛋白,達到同時對”多個樣本”進行大規模的蛋白定量。

  • 非標靶代謝體學分析

    非標靶代謝體學分析

    非標靶代謝體學 (Untargeted Metabolomics) 是藉由比較對照組和實驗組的代謝物,以尋找其代謝路徑差異的一種方法。這些差異有助於尋找臨床生物標記,應用在疾病的早期診斷或藥物的靶點發現及疾病的致病機轉研究等。

  • 微生物體16S擴增子定序 (16S Amplicon Sequencing)

    微生物體16S擴增子定序 (16S Amplicon Sequencing)

    16S rRNA為原核生物核糖體小亞基的重要組成,序列包含數個保守區域和9個高變區域,其中高變區具有屬或種的特異性,被認為是最適於細菌系統發育和分類鑑定的指標。因此對該區域中的序列進行定序已是研究環境微生物多樣性及群落組成差異的重要策略之一。

  • RNA Sequencing (Quantification)

    RNA Sequencing (Quantification)

    此項RNA定序技術是針對樣本中所有mRNA做高通量定序分析,通常定序結果的涵蓋率(coverage)和深度(depth)是研究人員最關心的兩樣條件。mRNA定序的結果可以顯示基因的轉錄表現量。

新知分享-部落格:『糞菌移植』(FMT, Fecal Microbiota Transplantation)近幾年不論在研究或是臨床上都已如火如荼開展中,而專注於微生物治療或基因檢測公司也越來越多,或許這正預告著『精準微生物醫療』的時代即將來臨。
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