• TOOLSignal boosting buffer

    TOOLSignal boosting buffer

    TOOLSignal Boosting Buffer is the best alternative for conventional blocking buffer and it improves the performance of your Western blot experiments for all types of antibodies. Simply incubate this buffer with primary or secondary antibodies without using any blocking buffer (milk or BSA) to obtain effective blocking result. TOOLSignal Boosting Buffer shortens experimental time and reduces the use of antibodies without compromising Western Blot signal intensity.

  • ToolsQuant II Fast RT Kit

    ToolsQuant II Fast RT Kit

       ToolsQuant II Fast RT Kit is designed for the first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). To avoid the contamination of gDNA, the kit contains gDNA Eraser that effectively remove gDNA (42°C, 3 min). It takes only 15 min (42°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.

  • Locking PLGEL Heavy, 1.5ml

    Locking PLGEL Heavy, 1.5ml

    Locking PLGEL (PLG) provides increased protection and ease of handling when working with standard organic extraction mixtures. The use of PLG can result in the recovery of 20 to 30% more nucleic acid than with traditional methods. After organic extraction, it is often difficult to recover nucleic acid in the aqueous, upper phase that is free from the denatured protein present at the aqueous and organic phase interface. PLG present during Phenol, Phenol:Chloroform, and Chloroform:Isoamyl Alcohol extractions migrates under centrifugal force to form a seal between the organic and aqueous phases. The organic phase and the interface material are effectively trapped below the PLG. The barrier is sufficiently durable that the aqueous upper phase, containing the nucleic acid, can then be recovered quantitatively by simply decanting or pipetting to a fresh tube. PLG is inert, heat stable, and does not interfere with standard nucleic acid restriction and modification enzymes. In fact, many of the reactions can be carried out in the presence of PLG at the appropriate temperature and then terminated by extraction with Phenol or Phenol:Chloroform. PLG can be present during the heat inactivation of enzymes (65°C for 10 minutes) prior to the organic extraction. The ability of PLG to separate the phases is based on the density differences of the aqueous and organic media. The organic layer must have a higher density than the PLG and aqueous phase, and the PLG must have a higher density than the aqueous phase. High salt and protein concentrations in the aqueous phase may affect both aqueous and organic phase densities. Different organic phase formulations also vary in density. When choosing a type of PLG, the composition of all components must be taken into consideration. For optimum phase separation, the compositions of the aqueous phase, organic phase, and PLG must be compatible. As a result, PLG is offered in two different density formulations: Light and Heavy.

  • TOOLSmart RNA Extractor

    TOOLSmart RNA Extractor

     TOOLSmart RNA Extractor is developed based on regular nucleic acid extraction Reagent with the addition of indicator. It has better lysis ability and higher sensitivity. It could be used to isolate total RNA from samples like virus, bacteria, plant tissue and body fluid. It maintains the integrity of RNA while disrupting cells and dissolving cell components during sample homogenization. TOOLSmart RNA Extractor can be applied to isolate RNA from both small amount of samples (50-100 mg tissue or 5 × 106cells) and large amount of samples (≥1 g tissue or ≥107cells) of human, animal, plant or bacteria, within one hour. DNA and protein contamination is eliminated thoroughly from RNA products. Isolated RNA can be used in Northern Blot, Dot Blot, poly(A) selection, in vitro translation, RNase protection assay and molecular cloning.

  • 細胞株基因編輯服務


    圖爾思生物科技公司為台灣最早開始推展CRISPR/Cas9技術且為最先開始利用此系統提供客製化的基因編輯細胞株。我們利用plasmid based系統,經驗豐富的sgRNA設計,活性高、脫靶率(off target)極低,並搭配獨一無二的surrogate reporter plamid,更快速幫客戶篩出KO/KI細胞株。

  • 小鼠基因編輯(CRISPR/Cas9 system)服務

    小鼠基因編輯(CRISPR/Cas9 system)服務

    圖爾思生物科技公司為台灣最早開始推展CRISPR/Cas9技術且為最先開始利用此系統提供客製化的基因編輯小鼠與大鼠。我們利用in vitro transcripted sgRNA與獨有的Cas9 protein,在單一個受精卵時期用microinjection方式將材料打入,六至八週九可以產出F0小鼠。

  • 蛋白質身份鑑定




  • 動植物基因體重定序 Resequencing (Animal & Plants)

    動植物基因體重定序 Resequencing (Animal & Plants)


  • 16S擴增子定序 (16S Amplicon Sequencing)

    16S擴增子定序 (16S Amplicon Sequencing)

    16S rRNA為原核生物核糖體小亞基的重要組成,序列包含數個保守區域和9個高變區域,其中高變區具有屬或種的特異性,被認為是最適於細菌系統發育和分類鑑定的指標。因此對該區域中的序列進行定序已是研究環境微生物多樣性及群落組成差異的重要策略之一。

  • RNA Sequencing (Quantification)

    RNA Sequencing (Quantification)


  • 全基因體定序 Whole Genome Sequencing (WGS)

    全基因體定序 Whole Genome Sequencing (WGS)


  • 全外顯子體定序 Whole Exome Sequencing (WES)

    全外顯子體定序 Whole Exome Sequencing (WES)

    針對基因體中表現蛋白質的部份(Exon)定序。以人類罕見遺傳疾病(Mendelian disorder)研究為例,只有不到2%的基因體屬外顯子,然而大約85%的疾病和exon變異有關聯。利用全外顯子體定序找出關鍵性變異的exon,相比於全基因體定序,定序成本較為經濟且更有意義

新知分享-部落格:『糞菌移植』(FMT, Fecal Microbiota Transplantation)近幾年不論在研究或是臨床上都已如火如荼開展中,而專注於微生物治療或基因檢測公司也越來越多,或許這正預告著『精準微生物醫療』的時代即將來臨。
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