Real-Time PCR Related Reagents

  • TOOLS 5X EvaGreen qPCR Mix with ROX

    TOOLS 5X EvaGreen qPCR Mix with ROX

    TOOLS 5X EvaGreen qPCR Mix with ROX is optimized for real-time quantitative PCR assays. The ready-to-load mix includes TOOLS HotTaq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye according to system requirements. Only water, template and primers need to be added. TOOLS HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

  • TOOLS 5X EvaGreen qPCR Mix

    TOOLS 5X EvaGreen qPCR Mix

    TOOLS 5X EvaGreen qPCR Mix with ROX is optimized for real-time quantitative PCR assays. The ready-to-load mix includes TOOLS HotTaq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye according to system requirements. Only water, template and primers need to be added. TOOLS HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

  • TOOLS 2X SYBR qPCR Mix

    TOOLS 2X SYBR qPCR Mix

    TOOLS 2xSYBR qPCR Mix Kit is specially designed to perform Real-time PCR in SYBR Green I fluorescent-based detection assays. The Real-Time PCR reaction buffer, a 2× pre-mixed solution included in this kit, provides an optimum concentration of SYBR Green I solution, which greatly facilitates the preparation of qPCR reaction mixture. TOOLS 2xSYBR qPCR Mix adopts a unique dual hot-start enzymes system (chemically modified HotStar Taq DNA polymerase and antibody modified Anti Taq DNA Polymerase), which, plus the pre-optimized buffer solution, provides a convenient format for highly sensitive and specific qPCR amplification.

  • Tools SuperFast SYBR qPCR Reagent

    Tools SuperFast SYBR qPCR Reagent

    Tools SuperFast SYBR qPCR Reagent is designed for SYBR Green I based quantitative PCR assays, enables fast and specific quantitative results. Optimized premix could reduce the running time and is suitable for regular and fast real-time PCR thermal cycler. 2X SuperFast SYBR qPCR Mix adopts antibody modified Anti Taq DNA polymerase. Combined with the unique PCR buffer, it could ensure a sensitive PCR detection on any Real-Time PCR thermal cycler. Total running time could be reduced by 60% compared by regular real-time PCR program. Meantime accurate quantification, high amplification efficiency, high specificity and wide credibility range could be achieved.

  • TOOLS Easy SYBR qPCR Mix

    TOOLS Easy SYBR qPCR Mix

    TOOLS Easy SYBR qPCR Mix kit is specially designed to perform Real-time PCR in SYBR Green I fluorescent-based detection assays. The Real-Time PCR reaction buffer, a 2× pre-mixed solution included in this kit, provides an optimum concentration of SYBR Green I solution, which greatly facilitates the preparation of qPCR reaction mixture. TOOLS 2xSYBR PreMix adopts a unique dual hot-start enzymes system (chemically modified HotStar Taq DNA polymerase and antibody modified Anti Taq DNA Polyerase), which, plus the pre-optimized buffer solution, provides a convenient format for highly sensitive and specific qPCR amplification.