RNA Ligase

  • T4 RNA Ligase 1

    T4 RNA Ligase 1

    T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded nucleic acids (RNA or DNA).

  • T4 RNA Ligase 2

    T4 RNA Ligase 2

    T4 RNA Ligase 2 catalyzes phosphodiester bond formation between a 5’ phosphate and 3’ hydroxyl of RNA. The preferred substrate is nicked double-stranded RNA but single-stranded RNA can also serve as a substrate. Ligation of single-stranded RNA substrates generates either intramolecular or intermolecular products. Besides nicked double-stranded RNA substrates, other nicked nucleic acids hybrids can be sealed. The strand containing the 5’ phosphate can either be DNA or RNA. The non-ligated strand of the duplex can be either RNA or DNA. T4 RNA ligase 2 requires ATP for activity unless the substrate is preadenylated on the 5’ end. A truncated version of T4 RNA ligase 2 is a better enzyme for preadenylated substrates because it generates less side-reaction ligation products than the full length enzyme.

  • T4 RNA Ligase 2, Truncated

    T4 RNA Ligase 2, Truncated

    T4 RNA Ligase 2 truncated catalyzes phosphodiester bond formation between a pre-adenylated 5’ phosphate (DNA or RNA) and the 3’ hydroxyl of RNA. The truncated enzyme contains the first 249 amino acids which makes the enzyme require a pre-adenylated 5’ terminal donor and eliminates the need for ATP. Because T4 RNA ligase 2 truncated cannot use the 5’ phosphate of RNA or DNA as a donor in the ligation reaction, it is useful for certain applications such as linker ligations with pre-adenylated 5’ DNA to 3’ hydroxyl RNA. The desired specific ligation products are enhanced dramatically over unwanted background ligation products, making the truncated enzyme superior to the full-length enzyme for this use.