T4 DNA Polymerase

  • T4 DNA Polymerase

    T4 DNA Polymerase

    T4 DNA Polymerase catalyzes the extension of a primed DNA
    template in the 5’→3’ direction. This enzyme exhibits a powerful 3’→5’ exonuclease activity, while lacking any inherent 5’→3’ exonuclease or strand displacement functions.

  • T4 Gene 32 Protein

    T4 Gene 32 Protein

    T4 Gene 32 Protein is a single-stranded DNA binding protein which is required for T4 DNA replication, recombination and repair. The protein has exhibited an ability to enhance the performance of several DNA synthesis-related activities, including DNA sequencing in secondary-structure rich regions and PCR amplification. T4 Gene 32 also greatly stimulates the rate of synthesis of T4 DNA Polymerase on primed-single-stranded substrates (5-10 fold increase in synthesis rate).

  • T4 Polynucleotide Kinase

    T4 Polynucleotide Kinase

    T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5’-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3’-monophosphate molecules (1). T4 PNK also exhibits 3’-phosphatase and 2’, 3’cyclicphosphodiesterase activities (2, 3, 4, 5, 6).