EasyPrep miRNA Extraction Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (from 20 nt ~200 nt), from cultured cells and various animal and human tissues. EasyPrep miRNA Extraction Kit combines phenol/guanidine-based lysis of samples and silica membrane - based purification of total RNA. Buffer TRO, included in the kit, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction. The Kit utilizes a silica-based two-column system to enrich small RNA from various sample sources. From sample lysate, RNAs greater than ~ 200 nt are bound to the first column. The flow-through, enriched with small RNA molecules, is mixed with a higher concentration of ethanol to increase the binding affinity of small RNA to the membranes of the second column. Bound small RNA molecules are then eluted, ready-to-use, and free of large RNA molecules that can inhibit expression analysis of small RNA molecules, Purified RNA is suitable for different downstream applications.
ToolmiRcute miRNA cDNA Synthesis Kit adds Poly(A) to the 3'-terminal of miRNA and synthesize the first-strand cDNA based on Poly(A) modified miRNA through oligo(dT)-universal tag primed reverse transcription. The kit contains all the necessary reagents in the process of adding Poly(A) and reverse transcription. The kit canprovide high efficient Poly(A) modification and reverse transcription and efficiently synthesize the first-strand cDNA from 20 pg-2 μg total RNA.
ToolmiRcute miRNA qPCR Detection Kit is designed for miRNA real-time PCR by using SYBR GreenI. The kitis composed of 2×miRcute miRNA Premix, 50× ROX Reference Dye and Reverse Primer (10 µM). 2×ToolmiRcute miRNApremix(withSYBRand ROX) was developed specifically for quantitative detection of miRNA. The DNA polymerase in this kit is the antibody modified hot-start DNA polymerase, which ensures high specificity, sensitivity and accurate quantitative detection in a wide range combined with the unique buffer.
Note: The kit has to be used with ToolmiRcute miRNA cDNA Synthesis Kit (Cat. no. TGKRB01)