Plasmid Extraction Kit

  • TOOLS Plasmid Mini kit

    TOOLS Plasmid Mini kit

    TOOLS Plasmid Mini kit is based on alkaline lysis technology followed by adsorption of DNA onto silica membrane in the presence of high salt. Plasmid DNA purified with this product is immediately ready for use. Phenol extraction and ethanol precipitation are not required. High-quality plasmid DNA is eluted in a small volume of Tris Buffer or deionized water. This protocol is designed for purification of up to 30 μg of plasmid DNA (<12kb) from 1-4 ml overnight cultures of E. coli in LB (Luria-Bertani) medium. Plasmid DNA prepared by this kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in vitro translation, and transfection of robust cells.

  • EasyPrep High Pure Plasmid Mini Plus Extraction kit

    EasyPrep High Pure Plasmid Mini Plus Extraction kit

    EasyPrep High Pure Plasmid Mini Plus Extraction kit is based on alkaline lysis technology followed by adsorption of DNA onto silica membrane in the presence of high salt. Plasmid DNA purified with this product is immediately ready for use. Phenol extraction and ethanol precipitation are not required. High-quality plasmid DNA is eluted in a small volume of Tris Buffer or deionized water. This protocol is designed for purification of up to 70 μg of plasmid DNA from 5-15 ml overnight culture of E. coli in LB (Luria-Bertani) medium.

    Plasmid DNA prepared by EasyPrep High Pure Plasmid Mini plus kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in vitro translation, and transfection of robust cells.

  • EasyPrep HY-Midi Plasmid Extraction Kit

    EasyPrep HY-Midi Plasmid Extraction Kit

    EasyPrep HY-Midi Plasmid Extraction Kit adopts specially designed buffer system that is used in conjunction with isopropanol precipitation to achieve fast and efficient purification of high pure plasmid DNA. Plasmid DNA prepared by this Kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in vitro translation, and transfection of cell lines.

    Recommended bacterial culture volume: 500-1500 ug plasmid with 100 ml bacterial culture for high-copy vectors; and 200-400 ug plasmid with 200 ml bacterial culture for low- copy vectors.

  • EasyPrep EndoFree Maxi Plasmid Extraction Kit

    EasyPrep EndoFree Maxi Plasmid Extraction Kit

    EasyPrep EndoFree Maxi Plasmid Extraction kit can be stored dry at room temperature (15-25°C) for up to 12 months without showing any reduction in performance and quality. For longer storage, it can be stored at 2-8°C. If any precipitate forms in the buffers after storage at 2-8°C, it should be dissolved by warming the buffers to 37°C before use. RNaseA (100mg/ml) can be stored for one year at room temperature (15-25°C). After adding RNaseA, Buffer P1 is stable for 6 months at 2-8°C.

  • EasyPrep Yeast Plasmid DNA Kit

    EasyPrep Yeast Plasmid DNA Kit

    TOOLS Yeast Plasmid DNA Kit is based on silica membrane technology and special buffer system for extraction of plasmid DNA from yeast sample. The spin column made of new type silica membrane can bind DNA optimally on given salt and pH conditions. Simple centrifugation processing completely removes contaminants and enzyme inhibitors such as proteins and divalent cations. The isolated plasmid DNA is suitable for restriction analysis PCR amplification, bacterial transformation and DNA sequencing. The protocol eliminates the need for phenol/chloroform extraction.