Enzymes & Molecular Biology Related Reagents

  • Protease Inhibitor Cocktail (EDTA free)

    Protease Inhibitor Cocktail (EDTA free)

    TOOLS protease inhibitor cocktail inhibits the degradation of proteins in tissue or cell extracts by endogenous proteases. It is useful for exploring particular processes that involve blocking the activity of specific proteases. This product can be used in mammalian cell lysates or tissue extracts to increase protein stability. As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates. TOOLS Protease Inhibitor Cocktail is a mixture of 5 pan-protease inhibitors for protection of protein integrity.

  • TOOLS Ethidium Bromide/Nuclease Eraser

    TOOLS Ethidium Bromide/Nuclease Eraser

    TOOLS Ethidium Bromide (EtBr)/ Nuclease Eraser is a stable/non- toxic solution effectively remove and inactivated EtBr and RNase/DNase. The eraser should be stored at room temperature. Incubate Eraser at 37°C if precipitates form. After contact with Eraser, if skin irritation or rash occurs, seek medical attention.

  • RNase inhibitor

    RNase inhibitor

      RNAse Inhibitor is an acidic, 52 kDa protein that is a potent non-competitive inhibitor of pancreatic-type ribonucleases such as RNase A, RNase B, and RNase C. The enzyme is provided as a fusion of the porcine RNAse Inhibitor gene with a proprietary, 22.5 kDa protein tag.


    Source of Protein

    A recombinant E. coli strain carrying the porcine RNAse Inhibitor gene.

     

  • Protease Inhibitor Cocktail (tablets)

    Protease Inhibitor Cocktail (tablets)

     Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of digesting the proteins present in the extract. An optimized method to improve the yield of native proteins is to add inhibitors of these enzymes known to be present in the source material. Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases. The cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates. TOOLS Protease Inhibitor is a blend of 5 pan-protease inhibitors for protection of protein integrity. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 actsagainst cystein proteases. 

    Leupeptin acts against both serine and cystein proteases.

  • DNA View

    DNA View

      TOOLS DNA view is an innovated nucleic acid stain to replace highly toxic ethidium bromide (EtBr) for detecting nucleic acid agarose gels. It emits green fluorescence when bound to DNA and RNA. TOOLS DNA view is as sensitive as EtBr and the staining protocol is also very similar, however, compared to EtBr, which is known as a strong mutagen, TOOLS DNA view causes much fewer mutations in the Ames test. In addition, it has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. Most importantly, it is not considered hazardous waste, can be disposed of according to standard laboratories procedures, and is stable for years.

  • 2.5 mM dNTP

    2.5 mM dNTP

     TOOLS dNTP contains premixed ready-to-use solution of dATP, dCTP, dGTP and dTTP (10 mM can be diluted with ddH2O in pH 7.5).

     

  • 10 mM dNTP

    10 mM dNTP

     TOOLS dNTP contains premixed ready-to-use solution of dATP, dCTP, dGTP and dTTP (10 mM can be diluted with ddH2O in pH 7.5).

     

  • 100mM dNTP Set (dATP,dTTP,dCTP,dGTP 0.5ml each)

    100mM dNTP Set (dATP,dTTP,dCTP,dGTP 0.5ml each)

     dNTP Set contains 0.5 ml of dATP, dCTP, dGTP and dTTP, (100 mM each in sterile deionized water in pH 7.5). This product is DNase and RNase free and suitable for all standard PCR techniques, including DNA sequencing, cDNA synthesis, primer extension, and nick translation.

     

  • Protease Inhibitor Cocktail

    Protease Inhibitor Cocktail

    Application

    Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of digesting the proteins present in the extract. An optimized method to improve the yield of native proteins is to add inhibitors of these enzymes known to be present in the source material. This phosphatase inhibitor cocktail is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly.

    Specificity        

    Serine protease, esterase, cysteine protease, metalloprotease, insulin-like protease, aminopeptidase, and aspartic acid protease, etc.

  • Phosphatase Inhibitor Cocktail

    Phosphatase Inhibitor Cocktail

    Phosphatase inhibitors are used when phosphorylation (activation) states of target proteins need to be studied and the phosphorylated residues of interest must remain intact. They are chemicals that aid in the extraction of intact proteins in their native modification state by inhibiting endogenous phosphatases that would otherwise dephosphorylate the proteins present in cell lysates and tissue extracts. Phosphatase Inhibitor Cocktail contains individual components with specific inhibitory properties to provide an all-around protection of the protein phosphorylation state. The six phosphatase inhibitors included in this mixture target a broad spectrum phosphatase categories. Dynamic protein phosphorylation is a key cellular signaling mechanism for cell processes regulation. When tissues are lysed to make whole cell extracts, the loss of natural compartmentalization causes normal regulation of cellular signaling to get distorted, and resident cell phosphatases within the cell extract are free to disorderly dephosphorylate proteins. The usual consequence of this unregulated state is biologically meaningless representation of protein activities (i.e. phosphorylation status) and false negative staining in anti-phosphoprotein immunostaining analyses. The addition of phosphatase inhibitors to the cell lysis buffer aids in the preservation of phosphorylated residues at the time of cell disruption. It is a Western blot related concentrated stock solution reagent containing a mixture of different phosphatase inhibitors that is to be added to cell lysis buffer to protect native phosphoproteins from dephosphorylation during proteins purification and sample preparation used in WB, Co-IP, ChIP, and protein kinase assays.

    Content:
    Proprietary mix of: Sodium fluoride, Sodium orthavanadium, Imidazole, Sodium molybdate, Sodium sulphate, Sodium pyrophosphate, B-phosphoric acid glycerol

    Target Specificity:
    Tyrosine phosphatase, acidic and alkaline phosphatase; Serine/threonine phosphatase, histidine phosphatase, etc.

  • High Sensitive BCA ProteinAssay Kit

    High Sensitive BCA ProteinAssay Kit

      The High Sensitive BCA Protein Assay Kitisadetergent-compatible bicinchoninic acidformulation for the colorimetric detectionandquantitation of total protein. An adaptation of theBCA Protein Assay Kit, the Micro BCA Kit hasbeen optimized for use with dilute proteinsamples (0.5-20μg/ml).The unique, patentedmethod utilizes bicinchoninic acid (BCA) as thedetection reagentfor Cu+1, which is formed whenCu+2is reduced byprotein inan alkalineenvironment.A purple-colored reaction product isformed by the chelation of two molecules of BCAwith one cuprousion (Cu+1). This water-solublecomplex exhibits a strong absorbance at 562 nmthat is linear with increasing proteinconcentrations.The macromolecular structure of protein, thenumber of peptide bonds andthe presence offour amino acids (cysteine, cystine, tryptophanand tyrosine) arereported to be responsible forcolor formation with BCA. Studies with di-, tri-andtetrapeptides suggest that the extent of colorformation is caused by more than the mere sumof individual color-producing functional groups.

       The Micro BCA Protein Assay Kit usesconcentrated reagents and a protocol that utilizesan extended incubation time at an elevatedtemperature (60°C, Test Tube Procedure only).The result is an extremely

  • BCA Protein Assay Kit

    BCA Protein Assay Kit

     The BCA Protein Assay is a detergent-compatible formulation based on bicinchoninicacid (BCA) for the colorimetric detection and quantization of total protein. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in analkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+1) using a unique reagent containing bicinchoninic acid. The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. This water-soluble complex exhibits a strong absorbance at 562 nm that is nearly linear within creasing protein concentrations over a broad working range(20-2,000 μg/ml). The BCA method is not a true end-point method; that is, the final color continues to develop. However, following incubation, the rate of continued color development is sufficiently slow to allow large numbers of samples to be assayed together. The macromolecular structure of protein, the number of peptide bonds and the presence of four particular amino acids (cysteine,cystine, tryptophan and tyrosine) are reported to be responsible for color formation withBCA.2Studieswith di-, tri-and tetrapeptides suggest that the extent of color formation caused by more than the mere sum of individual color producing functional groups. Accordingly, protein concentrations   generally are determined and reported with reference to standards of a common protein such as bovine serum albumin(BSA). A series of dilutions of known

  • RNasin

    RNasin

      RNasin (ribonucleases inhibitor) is a recombinant human placental protein (50 KD) which specifically inhibits RNases A, B and C. It does not effectively inhibit RNase 1, RNase T1, S1 Nuclease, RNase H, RNase, Taq DNA Polymerase, AMV, M-MuLV Reverse Transcriptases, and Phage RNA Polymerases (SP6, T7, or T3). RNasin is stable over a wide pH and temperature range and in1 mM dithiothreitol (DTT) solution.

    Applications

      RNasin inhibits the degradation of RNA by eukaryotic RNases in wide range of applications, including cDNA synthesis, RT-PCR, and in vitro transcription and translation reactions.

     

  • A-Tailing Reagent

    A-Tailing Reagent

      This reagent is suitable for PCR product that performed by Pfu DNA polymerase, it adds an adenine residue to the 3´-end of both strands of DNA for subsequent TA cloning.

     

  • Lysozyme

    Lysozyme

      This product is a ready-to-use solution. and has been purified from chicken egg white. This lysozyme solution can be used for the purification of both DNA and protein from bacteria.