RNAse Inhibitor is an acidic, 52 kDa protein that is a potent non-competitive inhibitor of pancreatic-type ribonucleases such as RNase A, RNase B, and RNase C. The enzyme is provided as a fusion of the porcine RNAse Inhibitor gene with a proprietary, 22.5 kDa protein tag.
Source of Protein
A recombinant E. coli strain carrying the porcine RNAse Inhibitor gene.
RNasin (ribonucleases inhibitor) is a recombinant human placental protein (50 KD) which specifically inhibits RNases A, B and C. It does not effectively inhibit RNase 1, RNase T1, S1 Nuclease, RNase H, RNase, Taq DNA Polymerase, AMV, M-MuLV Reverse Transcriptases, and Phage RNA Polymerases (SP6, T7, or T3). RNasin is stable over a wide pH and temperature range and in1 mM dithiothreitol (DTT) solution.
RNasin inhibits the degradation of RNA by eukaryotic RNases in wide range of applications, including cDNA synthesis, RT-PCR, and in vitro transcription and translation reactions.