Cell Proliferation Assay Kit

  • TOOLS Cell Counting-8 (CCK-8) kit

    TOOLS Cell Counting-8 (CCK-8) kit

    Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing Dojindo’s highly water-soluble tetrazolium salt. WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt]* produces a water-soluble formazan Dye upon reduction in the presence of an electron carrier. WST-8 is reduced by dehydrogenases in cells to give a yellow colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.

  • WST-1 Cell Proliferation and Cytotoxicity Assay Kit

    WST-1 Cell Proliferation and Cytotoxicity Assay Kit

    A main application allows assessing the viability (cell counting) and the proliferation of cells (cell culture assays). It can also be used to determine cytotoxicity of potential medicinal agents and toxic materials since those agents would stimulate or inhibit cell viability and growth. The kit components are sufficient for performing up to 100 or 500 assays.

  • MTT Cell Proliferation and Cytotoxicity Assay Kit

    MTT Cell Proliferation and Cytotoxicity Assay Kit

    MTT Cell Proliferation Assay Kit provides a simple method for determination of cell number using standard microplate absorbance readers. Determination of cell growth rates is widely used in the testing of drug action, cytotoxic agents and screening other biologically active compounds. Several methods can be used for such determinations, but indirect approaches using fluorescent or chromogenic indicators provide the most rapid and large-scale assays.Among such procedures, the MTT assay developed by Mossman1 is still among one of the most versatile and popular assays. The MTT assay involves the conversion of the water-soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to an insoluble formazan.2-4 The formazan is then solubilized, and the concentration determined by optical density at 570 nm. The result is a sensitive assay with excellent linearity up to approximately 96 cells per well.

  • Trypan Blue StainingCell Viability Assay Kit

    Trypan Blue StainingCell Viability Assay Kit

    Trypan blue, as one of cell staining dyes, has been proved to be excluded by alive cells, while could stain dead cells to blue.Through an optical microscope, the staining process can be observed and the number of cells can be quantitative counted. But whether the cells are suicide or are destroyed, this cannot be judged by this stain. Trypan Blue Staining Cell Viability Assay Kit is developed on this working principle. Usually, the cell whose cytomembrane lost its integrity is considered as a dead cell. After staining with trypan blue, users can record the number of cells directly through microscope, or take a photo under microscope then record the number.In this way, the accurate cell livability can be quantified.