cDNA Synthesis Kit & RTase

  • ToolScript MMLV RTase

    ToolScript MMLV RTase

    ToolScript M-MLV is an RNA-dependent DNA polymerase and consists of a single subunit with a molecular weight of 71kDa. It can be used in cDNA synthesis with RNA or RNA: DNA hybrids as templates. ToolScript M-MLV is the preferred reverse transcriptase for long mRNA templates (>5kb), since its RNase H activity is weaker than commonly used reverse transcriptase. It greatly reduces the degradation of RNA templates and therefore increases the productivity.

  • ToolsQuant II Fast RT Kit

    ToolsQuant II Fast RT Kit

       ToolsQuant II Fast RT Kit is designed for the first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). To avoid the contamination of gDNA, the kit contains gDNA Eraser that effectively remove gDNA (42°C, 3 min). It takes only 15 min (42°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.

  • ToolScript MMLV RT Kit

    ToolScript MMLV RT Kit

       ToolScript RT Kit is designed for the first strand cDNA synthesis from very tiny amount of total RNA or poly (A) +RNA for two-step RT-PCR. The kit is applied combined with PCR in detection of expression of rare genes, quantitative expression level of mRNA in extremely small number of cells and cloning the cDNA of the specific gene.

  • Tools FastCell cDNA Kit

    Tools FastCell cDNA Kit

      Tools FastCell cDNA Kit provides a high-speed procedure for generating first-strand cDNA directly from cultured cells. No RNA purification or RNase H digestion steps are necessary, minimizing pipetting tasks. Tools FastCell cDNA Kit supplies wash and lysis buffers for preparing lysates and stabilizing RNA; gDNA Eraser for eliminating genomic DNA contamination, and all reaction components for fast and efficient cDNA synthesis.

    Cell numbers and buffer volumes needed for different plates

  • ToolsQuant RT Kit

    ToolsQuant RT Kit

    ToolQuant RT Kit is designed for the first strand cDNA synthesis from tiny amount of total RNA or mRNA for two-step RT-PCR. ToolQuant Reverse Transcriptase is a new, unique enzyme, different from the reverse transcriptases of Moloney murine leukemia virus (MMLV) or avian myeloblastosis virus (AMV). ToolQuant Reverse Transcriptase is a recombinant heterodimeric enzyme expressed in E. coli. ToolQuant Reverse Transcriptase has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, leading to high yields of cDNA.

  • TOOLS Easy Fast RT Kit

    TOOLS Easy Fast RT Kit

    TOOLS Easy Fast RT Kit is designed for fast first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). The kit contains RT Enzyme, RNase Inhibitor, Random primers, Oligo dT primer, dNTP Mixture and reaction buffer. It takes only 15 min (42°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.