cDNA Synthesis Kit & RTase

  • ToolScript MMLV RTase

    ToolScript MMLV RTase

    ToolScript M-MLV is an RNA-dependent DNA polymerase and consists of a single subunit with a molecular weight of 71kDa. It can be used in cDNA synthesis with RNA or RNA: DNA hybrids as templates. ToolScript M-MLV is the preferred reverse transcriptase for long mRNA templates (>5kb), since its RNase H activity is weaker than commonly used reverse transcriptase. It greatly reduces the degradation of RNA templates and therefore increases the productivity.

  • ToolQuant One Step RT-PCR Kit

    ToolQuant One Step RT-PCR Kit

       RT-PCR is the technology by which RNA template is reversely transcribed to cDNA by the transcriptase and then target fragment is obtained by amplification using PCR based on the cDNA template. RT-PCR is applied in detection of level of genomics in cells, tissues, virus and clonging the cDNA of the specific gene. The ToolQuant One Step RT-PCR Kit contains a specially formulated enzyme blend for both reverse transcription and PCR. Only one reaction mix needs to be set up: no additional reagents need to be added after the reaction starts, which avoids contaminations and enhances sensitivity of detection. Although all of the enzymes are present in the reaction mix, the use of Hotmix Taq polymerase ensures the temporal separation of reverse transcription and PCR, allowing both processes to be performed sequentially in a single tube. ToolQuant One Step RT-PCR Kit contains unique reaction buffers which ensure the high enzymatic activity of the ToolQuant RTase and Hotmix Taq polymerase.

  • ToolsQuant II Fast RT Kit

    ToolsQuant II Fast RT Kit

       ToolsQuant II Fast RT Kit is designed for the first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). To avoid the contamination of gDNA, the kit contains gDNA Eraser that effectively remove gDNA (42°C, 3 min). It takes only 15 min (42°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.

  • Virus Super One Step RT-PCR Kit

    Virus Super One Step RT-PCR Kit

       Virus Super One Step RT-PCR Kit provides a convenient format for highly sensitive and specific RT-PCR. The kit contains combines first-strand cDNA synthesis and PCR amplification in the same tube. The One Step Enzyme Mix contains a specially formulated enzyme blend for both reverse transcription and PCR. Quant RTase is included in One Step Enzyme Mix and ensures highly efficient and specific reverse transcription. The RTase exhibits a higher affinity for RNA, facilitating transcription through secondary structures that inhibit other reverse transcriptases. Hot Start DNA Polymerase included in One Step Enzyme Mix provides hot-start PCR for highly specific amplification. During reverse transcription, chemically modified Hot Start DNA Polymerase is completely inactive and does not interfere with the reverse-transcriptase reaction. After reverse transcription, reactions are heated to 95°C for 15 min to activate Hot Start DNA Polymerase and to simultaneously inactivate the reverse transcriptases. This hot-start procedure using Hot Start DNA Polymerase eliminates extension from nonspecifically annealed primers and primer–dimers in the first cycle ensuring highly specific and reproducible PCR. The 5× One Step RT-PCR Buffer is designed to enable both efficient reverse transcription and specific amplification. The buffer contains reaction buffer, dNTP and One Step Enhancer Solution, which ensures high PCR efficiency in one step RT-PCR.

  • ToolScript MMLV RT Kit

    ToolScript MMLV RT Kit

       ToolScript RT Kit is designed for the first strand cDNA synthesis from very tiny amount of total RNA or poly (A) +RNA for two-step RT-PCR. The kit is applied combined with PCR in detection of expression of rare genes, quantitative expression level of mRNA in extremely small number of cells and cloning the cDNA of the specific gene.

  • Tools FastCell cDNA Kit

    Tools FastCell cDNA Kit

      Tools FastCell cDNA Kit provides a high-speed procedure for generating first-strand cDNA directly from cultured cells. No RNA purification or RNase H digestion steps are necessary, minimizing pipetting tasks. Tools FastCell cDNA Kit supplies wash and lysis buffers for preparing lysates and stabilizing RNA; gDNA Eraser for eliminating genomic DNA contamination, and all reaction components for fast and efficient cDNA synthesis.

    Cell numbers and buffer volumes needed for different plates

  • M-MuLV Reverse Transcriptase

    M-MuLV Reverse Transcriptase

     M-MuLV Reverse Transcriptase is a DNA polymerase which utilizes RNA as a substrate and exhibits no measurable proofreading 3′→5′ exonuclease function. This enzyme can perform cDNA synthesis by extending off a DNA primer annealed to an RNA template, or can copy a single-stranded DNA template.

     

    Source of Protein :

    A recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.

     

  • TOOLSmart RT Mix

    TOOLSmart RT Mix

      TOOLSmart RT Mix provides an efficient method of cDNA synthesis which is perfect for downstream Real Time PCR. The 2X pre-mixed reagent contains reverse transcriptase, random hexamers, oligo dT ,dNTP mixture and stabilizer. Simply add RNA and water into TOOLSmart RT Mix and the RT reaction can be completed in short time.