Protease/Phosphatase Inhibitor Cocktail

  • Protease Inhibitor Cocktail (EDTA free)

    Protease Inhibitor Cocktail (EDTA free)

    TOOLS protease inhibitor cocktail inhibits the degradation of proteins in tissue or cell extracts by endogenous proteases. It is useful for exploring particular processes that involve blocking the activity of specific proteases. This product can be used in mammalian cell lysates or tissue extracts to increase protein stability. As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates. TOOLS Protease Inhibitor Cocktail is a mixture of 5 pan-protease inhibitors for protection of protein integrity.

  • Protease Inhibitor Cocktail (tablets)

    Protease Inhibitor Cocktail (tablets)

     Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of digesting the proteins present in the extract. An optimized method to improve the yield of native proteins is to add inhibitors of these enzymes known to be present in the source material. Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases. The cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates. TOOLS Protease Inhibitor is a blend of 5 pan-protease inhibitors for protection of protein integrity. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 actsagainst cystein proteases. 

    Leupeptin acts against both serine and cystein proteases.

  • Protease Inhibitor Cocktail

    Protease Inhibitor Cocktail

    Application

    Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of digesting the proteins present in the extract. An optimized method to improve the yield of native proteins is to add inhibitors of these enzymes known to be present in the source material. This phosphatase inhibitor cocktail is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly.

    Specificity        

    Serine protease, esterase, cysteine protease, metalloprotease, insulin-like protease, aminopeptidase, and aspartic acid protease, etc.

  • Phosphatase Inhibitor Cocktail

    Phosphatase Inhibitor Cocktail

    Phosphatase inhibitors are used when phosphorylation (activation) states of target proteins need to be studied and the phosphorylated residues of interest must remain intact. They are chemicals that aid in the extraction of intact proteins in their native modification state by inhibiting endogenous phosphatases that would otherwise dephosphorylate the proteins present in cell lysates and tissue extracts. Phosphatase Inhibitor Cocktail contains individual components with specific inhibitory properties to provide an all-around protection of the protein phosphorylation state. The six phosphatase inhibitors included in this mixture target a broad spectrum phosphatase categories. Dynamic protein phosphorylation is a key cellular signaling mechanism for cell processes regulation. When tissues are lysed to make whole cell extracts, the loss of natural compartmentalization causes normal regulation of cellular signaling to get distorted, and resident cell phosphatases within the cell extract are free to disorderly dephosphorylate proteins. The usual consequence of this unregulated state is biologically meaningless representation of protein activities (i.e. phosphorylation status) and false negative staining in anti-phosphoprotein immunostaining analyses. The addition of phosphatase inhibitors to the cell lysis buffer aids in the preservation of phosphorylated residues at the time of cell disruption. It is a Western blot related concentrated stock solution reagent containing a mixture of different phosphatase inhibitors that is to be added to cell lysis buffer to protect native phosphoproteins from dephosphorylation during proteins purification and sample preparation used in WB, Co-IP, ChIP, and protein kinase assays.

    Content:
    Proprietary mix of: Sodium fluoride, Sodium orthavanadium, Imidazole, Sodium molybdate, Sodium sulphate, Sodium pyrophosphate, B-phosphoric acid glycerol

    Target Specificity:
    Tyrosine phosphatase, acidic and alkaline phosphatase; Serine/threonine phosphatase, histidine phosphatase, etc.

  • Phosphatase Inhibitor Cocktail II

    Phosphatase Inhibitor Cocktail II

    Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of modifying the proteins present in the extract. The best way to imporve the yield of native proteins is to add inhibitors of these aneymes known to be present in the source material. This phosphatase inhibitor cocktail has been optimized and tested for tyrosine protein phosphatases, acid and akaline phosphatase.

  • Phosphatase Inhibitor Cocktail III

    Phosphatase Inhibitor Cocktail III

    Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of modifying the proteins present in the extract. The best way to imporve the yield of native proteins is to add inhibitors of these aneymes known to be present in the source material. This phosphatase inhibitor cocktail has been optimized and tested for serine and threonine phosphatases, acid and alkaline phosphatase.