Assistant Reagents

  • RIPA Solution

    RIPA Solution

    Extraction of cellular proteins requires efficient cell lysis and protein solubilization while avoiding protein degradation and/or interference with protein immunoreactivity and biological activity. RIPA (Radio-Immunoprecipitation Assay) Solution enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. It has long been a widely used lysis and washed solution for small-scale affinity pull-down applications, such as immunoprecipitation since most antibodies and protein antigens are not adversely affected by the components of this solution. In addition,

    RIPA Solution minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions. RIPA Solution is supplied as a ready to use solution that requires no preparation. Protease and phosphatase require no preparation. Protease and phosphatase inhibitors may be added to the lysis solution as needed.

  • Cell lysis buffer

    Cell lysis buffer

    For EasyPrep HY Genomic DNA Extraction Kit.

  • Protein Precipitation Buffer

    Protein Precipitation Buffer

    For EasyPrep HY Genomic DNA Extraction Kit.

  • DNA Dissolving Buffer

    DNA Dissolving Buffer

    For EasyPrep HY Genomic DNA Extraction Kit.

  • RBC lysis buffer

    RBC lysis buffer

    For EasyPrep HY Genomic DNA Extraction Kit.

  • Haematoxylin
  • DAPI staining solution

    DAPI staining solution

    DAPI (4',6-diamidino-2-phenylindole) is a kind of fluorescent dye which can bind DNA strands robustly, and the fluorescence can be detected by fluorescence microscope. DAPI can dye live cells and fixed cells as it can transmit the whole membrane. The molecular formula is C16H15N5·2HCl with 350.25 molecular weight, CAS Number 28718-90-3.

    DAPI could transmit cell membrane and bind the double-strand DNA in the nucleus, and produce 20 times stronger fluorescence than itself. The sensitivity for double stranded DNA staining is many times larger comparing with EB. Blue fluorescent cell would be seen under the microscope. The efficiency detected by fluorescence microscope is very high(almost 100%), and there is no side effect for the live cells. DAPI staining is usually used in cell death detection. After staining with DAPI, detect with fluorescence microscope or flow cytometry. DAPI is also usually used in nucleus staining and double-strand DNA staining in some special situation. After heat treatment, using DAPI stain the cell for 3min, then morphological changes of the nucleus can be detected the under the fluorescence microscope.

    The largest excitation wavelength for DAPI is 340nm, and the largest emission wavelength is 488nm; When DAPI binds with double-strand DNA, the largest excitation wavelength is 360nm, while the largest emission wavelength becomes 460nm.

    This DAPI Staining Solution can be used for fixed cells or nucleus of tissue staining directly.

  • TOOLS Tris-Glycine-SDS 10x Buffer

    TOOLS Tris-Glycine-SDS 10x Buffer

    TOOLS Tris-Glycine-SDS is a biotechnology grade 10X Solution. A single strength (1X) solution contains 0.025 Tris base, 0.192 M glycine and 0.1% sodium dodecyl sulfate.

  • 10x PBS Buffer 1L STERILE

    10x PBS Buffer 1L STERILE

    TOOLS 10X PBS is a pH-adjusted phosphate buffers and saline solutions commonly used in biological research, it is diluted to a 1X working concentration (pH should be adjusted)

  • 10X TBS Buffer, 1L
  • TG-10X buffer
  • BSA Solution 10mg/ml
  • Tris Buffer 1.5M pH8.8
  • 4x Upper Buffer

    4x Upper Buffer

    TOOLS 4X Upper Buffer consist with 0.5M Tris (pH 6.8) and 0.4% SDS at the optimal concentration for convenient and quick preparation of polyacrylamide gels for protein analysis by SDS-PAGE. Dilution of 4X buffer to a 1X solution (0.125M Tris and 0.1% SDS) can be used in the preparation of the stacking gel for SDS-PAGE.

  • TAE50X buffer