This reagent is suitable for PCR product that performed by Pfu DNA polymerase, it adds an adenine residue to the 3´-end of both strands of DNA for subsequent TA cloning.
Poly(A ) Polymerase catalyzes the addition of AMP from ATP to the 3’ hydroxyl of RNA. The reaction requires Mg2+and is template independent.
Source of Protein :
The gene encoding E. coli Poly(A) Polymerase expressed from
a plasmid in E. coli.