RT-PCR is the technology by which RNA template is reversely transcribed to cDNA by the transcriptase and then target fragment is obtained by amplification using PCR based on the cDNA template. RT-PCR is applied in detection of level of genomics in cells, tissues, virus and clonging the cDNA of the specific gene. The ToolQuant One Step RT-PCR Kit contains a specially formulated enzyme blend for both reverse transcription and PCR. Only one reaction mix needs to be set up: no additional reagents need to be added after the reaction starts, which avoids contaminations and enhances sensitivity of detection. Although all of the enzymes are present in the reaction mix, the use of Hotmix Taq polymerase ensures the temporal separation of reverse transcription and PCR, allowing both processes to be performed sequentially in a single tube. ToolQuant One Step RT-PCR Kit contains unique reaction buffers which ensure the high enzymatic activity of the ToolQuant RTase and Hotmix Taq polymerase.
Virus Super One Step RT-PCR Kit provides a convenient format for highly sensitive and specific RT-PCR. The kit contains combines first-strand cDNA synthesis and PCR amplification in the same tube. The One Step Enzyme Mix contains a specially formulated enzyme blend for both reverse transcription and PCR. Quant RTase is included in One Step Enzyme Mix and ensures highly efficient and specific reverse transcription. The RTase exhibits a higher affinity for RNA, facilitating transcription through secondary structures that inhibit other reverse transcriptases. Hot Start DNA Polymerase included in One Step Enzyme Mix provides hot-start PCR for highly specific amplification. During reverse transcription, chemically modified Hot Start DNA Polymerase is completely inactive and does not interfere with the reverse-transcriptase reaction. After reverse transcription, reactions are heated to 95°C for 15 min to activate Hot Start DNA Polymerase and to simultaneously inactivate the reverse transcriptases. This hot-start procedure using Hot Start DNA Polymerase eliminates extension from nonspecifically annealed primers and primer–dimers in the first cycle ensuring highly specific and reproducible PCR. The 5× One Step RT-PCR Buffer is designed to enable both efficient reverse transcription and specific amplification. The buffer contains reaction buffer, dNTP and One Step Enhancer Solution, which ensures high PCR efficiency in one step RT-PCR.