T7 DNA Polymerase

  • T7 DNA Polymerase

    T7 DNA Polymerase

      T7 DNA Polymerase is the mesophilic, highly processive, replicative DNA polymerase from bacteriophage T7 that is responsible for the rapid and accurate replication of the virus' genome during its infection cycle. T7 DNA Polymerase is a two subunit protein, consisting of a polymerase domain (gene 5 from the T7 bacteriophage) and a processivity factor (E. coli trxA gene thioredoxin) (1,2). The enzyme possesses a powerful (3'→5') nuclease activity that acts on both single and double stranded DNA and appears to be responsible for the high fidelity of this enzyme and prevents strand displacement synthesis (3,4,5).