DNA Ligase

  • E. Coli DNA Ligase

    E. Coli DNA Ligase

     E. coli DNA ligase catalyzes the phosphodiester bond formation between an adjacent 5’ phosphate and a 3’ hydroxyl of DNA ends, requiring NAD+and Mg2+as cofactors. Ligation of blunt ended DNA is extremely inefficient relative to cohesive DNA end ligation and nick sealing.

     

    Source of Protein : 

    The gene encoding E. coli DNA Ligase expressed from a plasmid in E. coli.

     

  • T4 DNA Ligase (Rapid)

    T4 DNA Ligase (Rapid)

     T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).

     

    Source of Protein : 

    A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.

     

  • T4 DNA Ligase

    T4 DNA Ligase

     T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).

     

    Source of Protein : 

    A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.