ToolScript M-MLV is an RNA-dependent DNA polymerase and consists of a single subunit with a molecular weight of 71kDa. It can be used in cDNA synthesis with RNA or RNA: DNA hybrids as templates. ToolScript M-MLV is the preferred reverse transcriptase for long mRNA templates (>5kb), since its RNase H activity is weaker than commonly used reverse transcriptase. It greatly reduces the degradation of RNA templates and therefore increases the productivity.
M-MuLV Reverse Transcriptase is a DNA polymerase which utilizes RNA as a substrate and exhibits no measurable proofreading 3′→5′ exonuclease function. This enzyme can perform cDNA synthesis by extending off a DNA primer annealed to an RNA template, or can copy a single-stranded DNA template.
Source of Protein :
A recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.