Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3’ hydroxyl terminus of single or double stranded DNA molecules. The presence of 1 mM Co2+ stimulates the tailing of the 3’-ends of DNA fragments. This construct is sold as an N-terminal truncation of the terminal transferase gene attached to an N-terminal fusion tag.
Source of Protein :
An E. coli strain that carries the cloned terminal transferase gene from calf thymus.