End-Repair Mix

  • End-Repair Mix

    End-Repair Mix

     The End-Repair mix converts DNA containing damaged or incompatible 5’- and/or 3’-protruding ends to 5 -phosphorylated, blunt-ended DNA.  This high-concentration formulation of the End-Repair Mix is compatible with applications requiring >1 microgram of DNA to be prepared for blunt-end ligation.  The conversion to blunt-ended DNA is accomplished by exploiting the 5’→3’ polymerase and 3’→5’exonuclease activities of T4 DNA Polymerase. T4 Polynucleotide Kinase ensures that the ends of the blunt-ended DNA fragments are 5’-phosphorylated for subsequent ligation by T4 DNA Ligase.

     

    Source of Protein : 

    Purified from strains of E. coli that express the recombinant T4 DNA Polymerase, and T4 Polynucleotide Kinase genes, respectively.

     

  • End-Repair Mix (Low Concentration)

    End-Repair Mix (Low Concentration)

      The End-Repair mix converts DNA containing damaged or incompatible 5’- and/or 3’-protruding ends to 5 -phosphorylated, blunt-ended DNA.  This high-concentration formulation of the End-Repair Mix is compatible with applications requiring >1 microgram of DNA to be prepared for blunt-end ligation.  The conversion to blunt-ended DNA is accomplished by exploiting the 5’→3’ polymerase and 3’→5’exonuclease activities of T4 DNA Polymerase. T4 Polynucleotide Kinase ensures that the ends of the blunt-ended DNA fragments are 5’-phosphorylated for subsequent ligation by T4 DNA Ligase.

     

    Source of Protein : 

    Purified from strains of E. coli that express the recombinant T4 DNA Polymerase, and T4 Polynucleotide Kinase genes, respectively.