TOOLS miRNA RT Kit
TOOLS miRNA RT Kit adopts an innovative RT enzyme and buffer system as the perfect first step of TOOLS miRNA RT-qPCR assay. Along with TOOLS miRNA RTqPCR primer/probe set, TOOLS miRNA RT Kit enables the RT reaction completed within only 15 mins, and provides reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template.
Mature miRNAs are a group of short non-coding RNA molecules of 18-25 nucleotides in length, govern various aspects of cellular development and pathological abnormalities. A single cDNA preparation of the miRNA can be used with a TOOLS miRNA RT kit, and then TOOLS Easy 2×Probe qPCR Mix comes in to quantify the expression of mature miRNAs rapidly and accurately.
產品特色
- 簡單：與TOOLS miRNA RT-qPCR primer/probe set 兼容，僅需常用RT步驟即可專一反轉錄miRNA
- 快速：RT反應僅需15分鐘完成。
- 準確：採用高效反轉錄酶和RNase抑製劑的配方
- 高效：僅需15 pg - 150 ng的RNA為樣本
實驗數據
| Amplification plots compare the TOOLS miRNA Assay system with the kit from Company A. Synthetic RNA in 10-fold dilution from 1 × 1010 to 1 × 104 copies/rxn was used as the template. Identical RT-qPCR programs were run using Applied Biosystems QuantStudio 12K Flex Real-Time PCR System to analyze hsa-miR-140-3p. The TOOLS kit outperformed the kit from Company A with higher detection sensitivity. |
相關產品
TOOLS miRNA RT-qPCR primer/probe set:
TOOLS miRNA RT-qPCR primer/probe set: Human
TOOLS miRNA RT-qPCR primer/probe set: Mouse
TOOLS miRNA RT-qPCR primer/probe set: Rat
TOOLS miRNA RT-qPCR primer/probe set: Other
TOOLS Easy 2xProbe qPCR Mix
Cat#TTC-QE13
This optimized ready-to-load mix contains dNTP/dUTP mix, Mg2+, modified hot-start DNA polymerase, dUTP/UDG anticontamination system, specific ROX Reference Dye, and all components necessary to be directly used for a robust and low-template qPCR with high sensitivity, specificity, and reliability. The dUTP/UDG anticontamination system included into the mix eliminates the product contamination of qPCR reactions. TOOLS Easy 2×Probe qPCR Mix also contains ROX Reference Dye suitable for all qPCR instruments, and no adjustments are required for the dye concentration in different instruments.
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Easy: All-in-one optimized master mix
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Specific: Premixed with dUTP/UDG anticontamination system
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High efficiency and stability
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ROX Reference Dye for fluorescent signal normalization and compatible with all qPCR instruments.
EasyPrep miRNA Extraction Kit
Cat#DPT-BE01
- Efficient: Completed within 1 hr
- Quality: silica-based system to yield high-purity small RNA product
- Flexible: Excellent lysis ability for various sample sources
