1. 擬真：Scaffold-base原理，細胞真實模擬in vivo生長模式

2. 穩定：不擠壓細胞骨架、胞器

3. 快速：專利配方，基質能快速凝膠化，品質穩定、操作便利

4. 簡單：專用基質溶解配方，輕鬆收集完整的Spheroids

Figure 1. The Trophoblast cells (3A-subE) were culture in TOOLS 3D culture Plus kit.
◼ Cell number:
    U bottom plate: 2x10^4 cells/well
    3D culture kit: 2x10^4 cells in 40 µL cell-hydrogel suspension mix/well
◼ Culture plate:
    U bottom plate: 96 well U bottom plate
    3D culture kit: 24 well flat bottom plate
◼ Scale bar: Obj. 40X, 20 µm
 

Figure 2. The kidney disease cells were cultured in TOOLS 3D culture Plus Kit. Kidney disease cells were cultured in hydrogel-based 3D culture materials for 4 weeks. Cells were treated with drug (Drug1) and refreshed medium twice a week. Analysis using F-actin (Green) to observe the spheroid structure. Nucleus stained with DAPI (Blue). Scale bar = 100 um.

Great growth and integrity of spheroids

Figure 3. HCT116 cells were cultured in 3D with TOOLS 3D Culture Plus kit for day 0, day 3, and day 7. The spheroids grew successfully, and the volumes of spheroids increased following days.


Figure 4. Huh 7 and A549 are cultured in 3D and their spheroids were collected with TOOLS 3D Culture Plus Kit.
 

Suitable for a variety of cell lines：