Protease Inhibitor Cocktail

Protease Inhibitor Cocktail

Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of digesting the proteins present in the extract. TOOLS Protease Inhibitor Cocktail (100X) is a Western blot related ready-to-use concentrated stock solution reagent containing a blend of seven protease inhibitors that is to be added to cell lysis buffer to protect the integrity and functionality of native cellular proteins against degradation by multiple classes of endogenous proteases during protein extraction and sample preparation procedures. The product is supplied as a 100X concentrated stock solution in a liquid format for improved accuracy, solubility, and ease of use in comparison to traditional tablets.

產品型號 規格 單價 庫存 購買數量
TAAR-BBI2 1ml - -
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產品特點
  1. Easy to use-100X solution

  2. EDTA included

  3. Economical-more cost effective

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Protease Inhibitor Component Information

Protease  Inhibitor Component
MW
Protease Family Targeted
Inhibition Type
Typical Working (1X) Conc.
Concentration in 100X cocktail
E-64
357.4
Cysteine proteases (papain, calpain,lysosomal cathepsins)
Irreversible
1 to 20μM
1.5mM
AEBSF-HCI
239.5
Serine proteases (trypsin, chymotrypsin, plasmin, trypsinogen, urokinase,  allikrein)
Irreversible
0.2 to 1.0mM 
100mM
Bestatin
308.4
Amino peptidases
Reversible
1 to 10μM
5mM
Leupeptin 
475.6
Serine and cysteine proteases
Reversible
10 to 100μM
2mM
Aprotinin
6511.5
Serine proteases (trypsin, chymotrypsin, plasmin, trypsinogen, urokinase, kallikrein)
Reversible
100 to 200nM
80μM
Pepstatin A 
685.9
Aspartic acid proteases (pepsin and rennin)
Reversible  
1 to 20μM
1mM
EDTA 
372.2
Metalloproteases (thermolysin and carboxypeptidase A)
Reversible
1 to 10mM
1mM
常見問題

Q1.客戶反應Protease inhibitor cocktail用DMSO回溶的時候效果不好,應該如何改善?

A:客戶調整為4度搭配Sharker後有大幅改善,同時建議可以使用短暫超音波震盪

 

Q2.Protease Inhibitor Cocktail可否用一碇溶在100ml的extract buffer裡面?

A:不行,建議按照Protocol操作,回溶在10ml的extract buffer裡面

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