Phosphatase Inhibitor Cocktail
Phosphatase inhibitors are used when phosphorylation (activation) states of target proteins need to be studied and the phosphorylated residues of interest must remain intact. They are chemicals that aid in the extraction of intact proteins in their native modification state by inhibiting endogenous phosphatases that would otherwise dephosphorylate the proteins present in cell lysates and tissue extracts. Phosphatase Inhibitor Cocktail contains individual components with specific inhibitory properties to provide an all-around protection of the protein phosphorylation state. The six phosphatase inhibitors included in this mixture target a broad spectrum phosphatase categories. Dynamic protein phosphorylation is a key cellular signaling mechanism for cell processes regulation. When tissues are lysed to make whole cell extracts, the loss of natural compartmentalization causes normal regulation of cellular signaling to get distorted, and resident cell phosphatases within the cell extract are free to disorderly dephosphorylate proteins. The usual consequence of this unregulated state is biologically meaningless representation of protein activities (i.e. phosphorylation status) and false negative staining in anti-phosphoprotein immunostaining analyses. The addition of phosphatase inhibitors to the cell lysis buffer aids in the preservation of phosphorylated residues at the time of cell disruption. It is a Western blot related concentrated stock solution reagent containing a mixture of different phosphatase inhibitors that is to be added to cell lysis buffer to protect native phosphoproteins from dephosphorylation during proteins purification and sample preparation used in WB, Co-IP, ChIP, and protein kinase assays.
Proprietary mix of: Sodium fluoride, Sodium orthavanadium, Imidazole, Sodium molybdate, Sodium sulphate, Sodium pyrophosphate, B-phosphoric acid glycerol
Tyrosine phosphatase, acidic and alkaline phosphatase; Serine/threonine phosphatase, histidine phosphatase, etc.
YANG, Wen-Bin, et al. Dehydroepiandrosterone Induces Temozolomide Resistance Through Modulating Phosphorylation and Acetylation of Sp1 in Glioblastoma. Molecular neurobiology, 2018, 1-13.