High Sensitive BCA ProteinAssay Kit
The High Sensitive BCA Protein Assay Kit is a detergent-compatible bicinchoninic acidformulation for the colorimetric detectionandquantitation of total protein. An adaptation of theBCA Protein Assay Kit, the Micro BCA Kit hasbeen optimized for use with dilute proteinsamples (0.5-20μg/ml).The unique, patentedmethod utilizes bicinchoninic acid (BCA) as thedetection reagentfor Cu+1, which is formed whenCu+2is reduced byprotein inan alkalineenvironment.A purple-colored reaction product isformed by the chelation of two molecules of BCAwith one cuprousion (Cu+1). This water-solublecomplex exhibits a strong absorbance at 562 nmthat is linear with increasing proteinconcentrations.The macromolecular structure of protein, thenumber of peptide bonds andthe presence offour amino acids (cysteine, cystine, tryptophanand tyrosine) arereported to be responsible forcolor formation with BCA. Studies with di-, tri-andtetrapeptides suggest that the extent of colorformation is caused by more than the mere sumof individual color-producing functional groups.
The Micro BCA Protein Assay Kit usesconcentrated reagents and a protocol that utilizesan extended incubation time at an elevatedtemperature (60°C, Test Tube Procedure only).The result is an extremely