TOOLS Cell Counting (CCK-8) kit

TOOLS Cell Counting (CCK-8) kit

TOOLS Cell Counting (CCK-8) kit is a ready-to-use solution without premixing of components. Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give a yellow colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.


產品型號 規格 單價 庫存 購買數量
TEN-CCK8 5ml (500 assays) -
TEN-CCK8-100 100ml (10000 assays) -
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產品特點
  1. One-bottle Solution: no premixing of components is required.

  2. Lower Toxicity: cells can be use for others experiments after CCK-8 assay.

  3. Hight Sensitivity: CCK-8 is the highest sensitive dye for cell-based assay.

  4. Many Applications: Can be used on cell proliferation, cell toxicity, drug selection experiments.

  5. Data Repeatability: results of the data has been confirmed many times and can be repeated.

產品原理

實驗數據

 

參考文獻
  1. Wu CP et al. "Sitravatinib Sensitizes ABCB1- and ABCG2-Overexpressing Multidrug-Resistant Cancer Cells to Chemotherapeutic Drugs." Cancers (Basel). (2020)

  2. Wu CP et al. "MY-5445, a phosphodiesterase type 5 inhibitor, resensitizes ABCG2-overexpressing multidrug-resistant cancer cells to cytotoxic anticancer drugs." Am J Cancer Res. (2020) 

  3. Tang KW et al. "Design, Synthesis, and Anti-Bacterial Evaluation of Triazolyl-Pterostilbene Derivatives." Int J Mol Sci. (2019)

  4. Wu CP et al. "SIS3, a specific inhibitor of Smad3 reverses ABCB1-and ABCG2-mediated multidrug resistance in cancer cell lines." Cancer letters 433 (2018): 259-272.

  5. Hsiao SH et al. "The FLT3 inhibitor midostaurin selectively resensitizes ABCB1-overexpressing multidrug-resistant cancer cells to conventional chemotherapeutic agents." Cancer letters 445 (2019): 34-44.

  6. Wu CP et al. "Avapritinib, a selective inhibitor of KIT and PDGFRα reverses ABCB1 and ABCG2-mediated multidrug resistance in cancer cell lines." Molecular pharmaceutics (2019).

  7. Wu CP et al. "Human ATP-binding cassette transporters ABCB1 and ABCG2 confer resistance to histone deacetylase 6 inhibitor ricolinostat (ACY-1215) in cancer cell lines." Biochemical pharmacology 155 (2018): 316-325.

  8. Hsiao SH et al. The positive inotropic agent DPI-201106 selectively reverses ABCB1-mediated multidrug resistance in cancer cell lines." Cancer letters 434 (2018): 81-90.

  9. Wu CP, Lusvarghi S, Hsiao SH, Liu TC, Li YQ, Huang YH, Hung TH, Ambudkar SV. Licochalcone A Selectively Resensitizes ABCG2-Overexpressing Multidrug-Resistant Cancer Cells to Chemotherapeutic Drugs. J Nat Prod. 2020 May 22;83(5):1461-1472.

  10. Wu CP, Hung TH, Hsiao SH, Huang YH, Hung LC, Yu YJ, Chang YT, Wang SP, Wu YS. Erdafitinib Resensitizes ABCB1-Overexpressing Multidrug-Resistant Cancer Cells to Cytotoxic Anticancer Drugs. Cancers (Basel). 2020 May 26;12(6):1366.

  11. Wu CP, Hung CY, Lusvarghi S, Huang YH, Tseng PJ, Hung TH, Yu JS, Ambudkar SV. Overexpression of ABCB1 and ABCG2 contributes to reduced efficacy of the PI3K/mTOR inhibitor samotolisib (LY3023414) in cancer cell lines. Biochem Pharmacol. 2020 Oct;180:114137.

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