ToolsQuant II Fast RT Kit

ToolsQuant II Fast RT Kit

   ToolsQuant II Fast RT Kit is designed for the first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). To avoid the contamination of gDNA, the kit contains gDNA Eraser that effectively remove gDNA (42°C, 3 min). It takes only 15 min (42°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.

產品型號 規格 單價 庫存 購買數量
KRT-BA06-2 100 rxns. - -
KRT-BA06 25 rxns. - -
加入購物車
產品特點
  1. High efficiency:  Reverse transcription efficiency above 95%.

  2. gDNA Eraser included:remove genomic DNA in 3min

  3. High performance:Tolerate to GC-rich template

  4. Fast: Only 21 min experimental procedure

操作流程

       

實驗數據

TOOLSQuant II Fast RT Competition Test:

參考文獻
  1. CHANG, Chih-Jung, et al. Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota. Nature communications, 2015, 6: 7489.

  2. Wu, Yu‐yi, et al. "Dcl2‐and Rdr6‐dependent transitive silencing of smxl4 and smxl5 in Arabidopsis dcl4 mutants causes defective phloem transport and carbohydrate over‐accumulation." The plant journal 90.6 (2017): 1064-1078.

  3. ​Hou, Cheng‐Yu, et al. "Global analysis of truncated RNA ends reveals new insights into ribosome stalling in plants." The plant cell (2016): tpc-00295.

  4. CHANG, Chih-Jung, et al. Antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice. International Journal of Obesity, 2018, 42.2: 231.​

常見問題

Q1.利用Quant II合成的cDNA進行qPCR後,結果相較於以前跑的數值CT值變高,是否正常?

A:最佳的RT反應RNA劑量為1ug,可以調整濃度後看看,並且請再確認Control和目標Gene的比值是否有差異

 

Q2.合成的cDNA效果不好,應該如何改善?

A:請再次確認目標基因的長度,Quant II最多合成長度為4kb。

 

Q3.Quant II RT反應,最後95C的步驟若沒有做,是否會有什麼影響?

A:為了將Enzyme inactive,建議要完成95C(3分鐘)的步驟。

 

Q4.Quant II的Primer成分及濃度是什麼?

A:包含Random primer及Oligo dT primer,濃度為1:1

 

Q5.Quant II可否用於Ecoli系統的RNA?

A:可以, Quant II所附的Primer mix是含有Random Primer,因此可以用於Ecoli系統

 

Q6.Quant II的input量低於50ng可以嗎?

A:建議不要,依protocol最低的建議量為50ng,除非受限樣本的情況下再進行嘗試

 

Q7.Quant II是否可以作流感病毒的RT?

A:可以, 有randon primer即可

 

Q8.若客戶使用Quant II時,希望僅使用Oligo dT或是random primer的情況下,Primer要使用多少濃度?

A:建議參考protocol說明,在final體積20ul的情況下,Oligo dT及Random Primer濃度為50pmol,則Specific Primer為5pmol

 

Q9.Quant II是否可以自行調整Buffer,以及不做gDNA去除的步驟?

A:若要調整不用去除gDNA的步驟請客戶自行嘗試,但Buffer的量還是請依照Datasheet中提到的gDNA removal reaction及RT reaction的比例調整

 

Q10.Qunat II中的Buffer是否含有Rnase Inhibitor?

A:有的

 

Q11.Mastermix裡面是否含有RNaseH?

A:無 

 

訂閱圖爾思電子報

您可以從電子郵件中得到我們最新的消息與資訊

訂閱服務確認

已發送 Email 驗證信給你,請點擊信件連結以完成訂閱程序

訂閱失敗

暫時無法接受訂閱,請稍候重新嘗試