EasyPrep Stool Genomic DNA Kit

EasyPrep Stool Genomic DNA Kit

EasyPrep Stool Genomic DNA Kit is based on silica membrane technology and special buffer system for stool sample gDNA extraction. The spin column made of new type silica membrane can bind DNA optimally on given salt and pH conditions. Simple centrifugation processing completely removes impurity, proteins, humic acid and other organic compounds. The high quality, high purity and full-length gDNA purified by this kit is ready for use in downstream applications such as PCR.

產品型號 規格 單價 庫存 購買數量
DPT-BC28 50preps - -
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產品特點

 

1. No phenol / chloroform extraction.

 

2. Consistent, high yield.

 

3. High-quality purified DNA can be obtained in 1 hour.

 

4. For NGS application/16S.

 

實驗數據

    

 

      Figure 1. Genomic DNA extracted from mouse stool

      using EasyPrep Marine DNA Kit. 

注意事項

1. Repeated freezing and thawing of stored samples should be avoided, since this leads to reduced DNA size and amount.

2. If precipitates formed in Buffer GSL or Buffer GB, warm buffer to 56°C until precipitates fully dissolve.

3. All centrifugation steps should be carried out in a conventional table-top micro centrifuge at room temperature.

常見問題

Q1.Stool extraction kit中Buffer GSL遇到有結晶的情況要怎麼辦?

A:此結晶情況係屬正常,客戶可以再使用前先用50C左右的溫水浴加溫,結晶情況即會消失,並且不影響Buffer效果

 

Q2.若是stool樣本很稀少,這樣是否還可以用Stool DNA extraction kit (DPT-BC28)做萃取?

A:麻煩客戶先確認樣本的重量,若真的比較稀少的情況下,建議少用Buffer based的Fecal Extracton kit (TX-STD01)進行萃取

 

Q3.若是僅有約90mg的Stool樣本,是否依樣可以用kit抽取?

A:可以的,且依照Protocol的步驟加入Buffer進行萃取即可

 

Q4.若是Stool的sample放在30%的Glycerol裡面,是否可以再用BC28的Kit進行萃取?

A:由於30%Glycerol會很黏稠,直接加入Lysis buffer的情況下可能會Lysis不完全,因此建議先用震盪均質機做均質,且均質的時間要足夠,再遵照Kit的Protocol做即可

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