ToolScript MMLV RT Kit

ToolScript MMLV RT Kit

   ToolScript RT Kit is designed for the first strand cDNA synthesis from very tiny amount of total RNA or poly (A) +RNA for two-step RT-PCR. The kit is applied combined with PCR in detection of expression of rare genes, quantitative expression level of mRNA in extremely small number of cells and cloning the cDNA of the specific gene.

產品型號 規格 單價 庫存 購買數量
TGKRA04 100preps -
加入購物車
產品特點
  1. Full-length fist strand cDNA up to 13kb.

  2. Optimum reaction temperature 42°C

  3. Complete kit: all the components for the RT reaction are included.

參考文獻
  1. Chu YD, Lin WR, Lin YH, Kuo WH, Tseng CJ, Lim SN, Huang YL, Huang SC, Wu TJ, Lin KH, Yeh CT. COX5B-Mediated Bioenergetic Alteration Regulates Tumor Growth and Migration by Modulating AMPK-UHMK1-ERK Cascade in Hepatoma. Cancers (Basel). 2020 Jun 22;12(6):1646. 

  2. Li CJ, Lin HY, Ko CJ, Lai JC, Chu PY. A Novel Biomarker Driving Poor-Prognosis Liver Cancer: Overexpression of the Mitochondrial Calcium Gatekeepers. Biomedicines. 2020 Oct 24;8(11):E451. 

  3. Wang WH, Yeh CS, Lin CY, Yuan RY, Urbina AN, Lu PL, Chen YH, Chen YA, Liu FT, Wang SF. Amino Acid Deletions in p6Gag Domain of HIV-1 CRF07_BC Ameliorate Galectin-3 Mediated Enhancement in Viral Budding. Int J Mol Sci. 2020 Apr 21;21(8):2910.

  4. Chen CY, Chen CC, Chuang WY, Leu YL, Ueng SH, Hsueh C, Yeh CT, Wang TH. Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells. Front Oncol. 2020 Feb 25;10:216. 

  5. Chu YD, Lai HY, Pai LM, Huang YH, Lin YH, Liang KH, Yeh CT. The methionine salvage pathway-involving ADI1 inhibits hepatoma growth by epigenetically altering genes expression via elevating S-adenosylmethionine. Cell Death Dis. 2019 Mar 11;10(3):240. 

  6. Huang JP, Hsu SC, Meir YJ, Hsieh PS, Chang CC, Chen KH, Chen JK, Hung LM. Role of dysfunctional adipocytes in cholesterol-induced nonobese metabolic syndrome. J Mol Endocrinol. 2018 May;60(4):307-321. 

  7. Chen CY, Fang JY, Chen CC, Chuang WY, Leu YL, Ueng SH, Wei LS, Cheng SF, Hsueh C, Wang TH. 2-O-Methylmagnolol, a Magnolol Derivative, Suppresses Hepatocellular Carcinoma Progression via Inhibiting Class I Histone Deacetylase Expression. Front Oncol. 2020 Aug 11;10:1319.

  8. Li CJ, Lin LT, Chu PY, Chiang AJ, Tsai HW, Chiu YH, Huang MS, Wen ZH, Tsui KH. Identification of Novel Biomarkers and Candidate Drug in Ovarian Cancer. J Pers Med. 2021 Apr 19;11(4):316.

  9. Li CJ, Chiu YH, Chang C, Chang YI, Sheu JJ, Chiang AJ. Acetyl Coenzyme A Synthase 2 Acts as a Prognostic Biomarker Associated with Immune Infiltration in Cervical Squamous Cell Carcinoma. Cancers (Basel). 2021 Jun 22;13(13):3125.

  10. Lin YH, Wu MH, Liu YC, Lyu PC, Yeh CT, Lin KH. LINC01348 suppresses hepatocellular carcinoma metastasis through inhibition of SF3B3-mediated EZH2 pre-mRNA splicing. Oncogene. 2021 Jul;40(28):4675-4685.

常見問題

Q1.MMLV和AMV的RT酵素適合使用溫度為多少?

A:兩者適合的溫度皆為42度C,目前我們沒有販售AMV的RT酵素

訂閱圖爾思電子報

您可以從電子郵件中得到我們最新的消息與資訊

訂閱服務確認

已發送 Email 驗證信給你,請點擊信件連結以完成訂閱程序

訂閱失敗

暫時無法接受訂閱,請稍候重新嘗試