CRISPR (clusters of regularly interspaced palindromic repeats)/Cas (CRISPR-associated) system is derived from an adaptive immune response against invading phages and plasmids in bacteria and archaea termed type II CRISPR. This genome editing technology is now widely used for making gene knockout/knock-in animals and plants as well as genome-modified cell lines. 

  TOOLS provides a custom CRIPSR/Cas system in which ready-to-use system for your gene/locus will be developed upon request. Our special CRISPR/Cas System has two separated plasmids, one is sgRNA over-expressing plasmid and the other is Cas9 protein over-expressing plasmid. So, you can adjust ratio of these two plasmids to reach the best effiency during transfecion. Our sgRNA are modified to increase the binding ability with target DNA.