TOOLSmart RNA Extractor

TOOLSmart RNA Extractor

 TOOLSmart RNA Extractor is developed based on regular nucleic acid extraction Reagent with the addition of indicator. It has better lysis ability and higher sensitivity. It could be used to isolate total RNA from samples like virus, bacteria, plant tissue and body fluid. It maintains the integrity of RNA while disrupting cells and dissolving cell components during sample homogenization. TOOLSmart RNA Extractor can be applied to isolate RNA from both small amount of samples (50-100 mg tissue or 5 × 106cells) and large amount of samples (≥1 g tissue or ≥107cells) of human, animal, plant or bacteria, within one hour. DNA and protein contamination is eliminated thoroughly from RNA products. Isolated RNA can be used in Northern Blot, Dot Blot, poly(A) selection, in vitro translation, RNase protection assay and molecular cloning.

產品型號 規格 單價 庫存 購買數量
DPT-BD24 100ml x2 - -
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產品特點
  1. High yield, rapid purification of high-quality RNA.

  2. Purify RNA within 1 hour.

  3. Suitable for any sample type.

  4. Low sample amount limitation.

  5. Retain short RNA fragment.

  6. High sensitivity, suitable for virus RNA extraction.

 

 

操作流程

實驗數據

      Total RNA extraction result

    

     Figure 1. RNA extracted from rat liver tissue
     using 1ml of TOOLSmart RNA Extractor. 
     Sample amount: 30mg of rat liver.
     3ul of 80ul eluted RNA were leaded per lane.

 

 

    

     Figure 2. RNA extracted from HepG2 cells
     using 1ml of TOOLSmart RNA Extractor. 
     Sample amount: 1x10^6 HepG2 cells.
     3ul of 30ul eluted RNA were leaded per lane.

 

 

    

    Figure 3. RNA extracted from Oryza sativa
    leaves using 1ml of TOOLSmart RNA 
    Extractor. Sample amount: 100mg.
    3ul of 50ul eluted RNA were loaded per lane.


      miRNA extraction result


    

   Figure 4. miRNA extracted from U937
    cells (8 x 10^6 cells)
    Lane1: 100bp marker
    Lane2: other brand
    Lane3: TOOLSmart RNA Extractor
常見問題

Q1. RNA extractor (200ml)共可以進行多少組織的RNA萃取?

A: 請參考Data Sheet使用,平均1ml RNA extractor可以抽取20~50mg的組織

 

Q2. RNA extractor是否可以萃取miRNA?

A: 可以,RNA extractor可以萃取所有片段大小的Total RNA (miRNA)

 

Q3.若是同時要抽脂肪、肌肉、肝、細胞等組織的RNA extraction是否有合適的kit?

A:若要同時符合這麼多種組織使用(由其脂質),建議還是選擇Smart Extractor比較好

參考文獻
  1. CHEN, Hui‐Feng; HSU, Chen‐Ming; HUANG, Yi‐Shuian. CPEB2‐dependent translation of long 3′‐UTR Ucp1 mRNA promotes thermogenesis in brown adipose tissue. The EMBO journal, 2018, 37.20: e99071.

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