TOOLS eSpCas9 (1.1) Protein

TOOLS eSpCas9 (1.1) Protein

  The CRISPR (clustered regularly interspaced short palindromic repeats) system is a prokaryotic adaptive immune system that consists of single guide RNA and Cas9 protein for performing genome editing in many organisms. 

TOOLS eSpCas9 (1.1) protein (K848A/K1003A/R1060A) is a purified nuclease from E coli using Cas9 gene of Streptococcus pyogenes. It is a ready-to-use product containing a nuclear localization signal (NLS) for genome editing experiments resulting site-specific cleavage of double-stranded DNA.

產品型號 規格 單價 庫存 購買數量
TS-cas901-50ug 50 ug - -
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產品特點

1. High purity & efficiency

2. High specificity

3. No off target

4. Suitabel for cell and animal experiment

活性測試

蛋白質純度測試:

  

         1ug protein, 7.5% SDS-PAGE

 

蛋白質活性測試:

        

             Lane 1: Supercoiled plasmid
             Land 2: Linear plasmid cut by Cas9 protein
             0.6% agarose gel

 

 

 

           50nM protein with 60nM sgRNA or 100nM protein with 12nM sgRNA
           100ng pGEM plasmid incubation 37˚C, 1hour
            0.6% agarose gel, 100V, 30min
            Target gene: zebrafish Fak1a
常見問題

Q1.是否能先從-80分裝部分出來使用? 放在4度冰箱暫存一周的話,效能會不會受影響?

A:可以分裝,4度C保存請於1-2天內使用完畢

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