Protease Inhibitor Cocktail (tablets)

Protease Inhibitor Cocktail (tablets)

 Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of digesting the proteins present in the extract. An optimized method to improve the yield of native proteins is to add inhibitors of these enzymes known to be present in the source material. Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases. The cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates. TOOLS Protease Inhibitor is a blend of 5 pan-protease inhibitors for protection of protein integrity. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 actsagainst cystein proteases. 

Leupeptin acts against both serine and cystein proteases.

產品型號 規格 單價 庫存 購買數量
TFU-T25 25 Tablets (EDTA-Free) - -
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產品特點
  1. Easy to use

  2. EDTA free-formulated without EDTA, a metalloproteinase inhibitor

  3. Economical-more cost-effective.

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TOOLS Protease inhibitor is blend of 5 pan-protease inhibitor for protection of protein integrity.

Component
Target
Inhibitor Type
AEBSF 
Serine proteases
Irreversible
Aprotinin
Serine proteases
Reversible
Bestatin
Aminopeptidases
Reversible
E-64
Cysteine proteases
Irreversible
Leupeptin
Serine and cysteine proteases
Reversible

 

常見問題

Q1.客戶反應Protease inhibitor cocktail用DMSO回溶的時候效果不好,應該如何改善?

A:客戶調整為4度搭配Sharker後有大幅改善,同時建議可以使用短暫超音波震盪

 

Q2.Protease Inhibitor Cocktail可否用一碇溶在100ml的extract buffer裡面?

A:不行,建議按照Protocol操作,回溶在10ml的extract buffer裡面

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