TOOLStripping Buffer

TOOLStripping Buffer

TOOLStripping Buffer is a ready-to-use buffer and it safely and effectively removes primary and secondary antibodies from western blot membranes. Simply incubate this buffer with PVDF or nitrocellulose membrane to allow Western blots to be re-probed with other antibodies.



產品型號 規格 單價 庫存 購買數量
TW-ST500 500 ml - -
加入購物車
產品特點
  1. Ready to use reagent.

  2. Effectively removes primary and secondary antibodies from western blot membranes.

  3. 3 min complete stripping procedure.    

  4. No stinky smelling (without addition of 2-ME)

  5. Non-hazardous solution

實驗數據
     
        Fig 1.Lane 1: marker, Lane 2: RD cells, Lane 3: Stau1-out RD cells
        Target protein: Stau1
        Protein input: 30ug
        Exposure time: 600S'
        1° antibody dilution 1:1000
        2° antibody dilution 1:5000
常見問題

Q1.為何Stripping buffer無法將一抗完全洗乾淨?

A:由於每個蛋白和抗體的affinity不同,因此Stripping的時間也必須調整,建議可先延長時間至30分鐘看看 

參考文獻
  1. CHANG, Jer-Hwa, et al. Downregulating CD26/DPPIV by apigenin modulates the interplay between Akt and Snail/Slug signaling to restrain metastasis of lung cancer with multiple EGFR statuses. Journal of Experimental & Clinical Cancer Research, 2018, 37.1: 199.

  2. LIN, Yang-Hsiang, et al. Thyroid hormone negatively regulates tumorigenesis through suppression of BC200. Endocrine-related cancer, 2018, 25.12: 967-979.

訂閱圖爾思電子報

您可以從電子郵件中得到我們最新的消息與資訊

訂閱服務確認

已發送 Email 驗證信給你,請點擊信件連結以完成訂閱程序

訂閱失敗

暫時無法接受訂閱,請稍候重新嘗試