Protease Inhibitor Cocktail (EDTA free)

Protease Inhibitor Cocktail (EDTA free)

TOOLS protease inhibitor cocktail inhibits the degradation of proteins in tissue or cell extracts by endogenous proteases. It is useful for exploring particular processes that involve blocking the activity of specific proteases. This product can be used in mammalian cell lysates or tissue extracts to increase protein stability. As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates. TOOLS Protease Inhibitor Cocktail is a mixture of 5 pan-protease inhibitors for protection of protein integrity.

產品型號 規格 單價 庫存 購買數量
TFU-PI 1ml - -
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產品特點
  1. Easy to use-100X solution

  2. EDTA free-formulated without EDTA, a metalloproteinase inhibitor

  3. Economical-more cost-effective

 

常見問題

Q1.客戶反應Protease inhibitor cocktail用DMSO回溶的時候效果不好,應該如何改善?

A:客戶調整為4度搭配Sharker後有大幅改善,同時建議可以使用短暫超音波震盪

 

Q2.Protease Inhibitor Cocktail可否用一碇溶在100ml的extract buffer裡面?

A:不行,建議按照Protocol操作,回溶在10ml的extract buffer裡面

 

參考文獻
  1. YANG, Wen-Bin, et al. Dehydroepiandrosterone Induces Temozolomide Resistance Through Modulating Phosphorylation and Acetylation of Sp1 in Glioblastoma. Molecular neurobiology, 2018, 1-13.

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