EasyPrep EndoFree Maxi Plasmid Extraction kit V2

EasyPrep EndoFree Maxi Plasmid Extraction kit V2

TOOLS Endotoxin Free Maxi Plasmid kit V2 is based on alkaline lysis technology followed by adsorption of DNA onto silica membrane in the presence of high salt. This product is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in vitro translation, and transfection of robust cells. With endotoxin removal buffer, the residual endotoxin is as low as <0.1 EU/μg.
Recommended bacterial culture volume: 500~1500μg plasmid with 100ml bacterial culture for high-copy vectors; and 50~300μg plasmid with 200ml bacterial culture for low- copy vectors.

產品型號 規格 單價 庫存 購買數量
DPT-BA20 10preps -
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產品特點
  1. Endotoxin free (<0.1 EU/ug)

  2. 500~1500ug plasmid for high copy vectors ; 50~300ug for low copy vectors

  3. Special buffer ER and CS1 column to remove endotoxin

  4. Excellent transfection efficiency

常見問題

Q1. 若是High Copy的Plasmid,萃取結果不佳時,如何提升萃取量?

A: 建議第做Elution步驟的時候,可以先將Elution Buffer加熱至55度,另外由於Membrane的面積比較大,所以Elution的步驟也可以重複進行2-3次,但不用每次都加熱

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