2x Ultra Hi-Fi PCR Master Mix

2x Ultra Hi-Fi PCR Master Mix

TOOLS 2× Ultra Hi-Fi PCR Master Mix is is a new generation superior enzyme based on Ultra Hi-Fi DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau-inhibiting factor newly added to Ultra Hi-Fi greatly improve its long-fragment amplification ability, specificity, and PCR yield. Ultra Hi-Fi is capable of amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of P Ultra Hi-Fi is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Ultra Hi-Fi has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. P Ultra Hi-Fi contains two monoclonal antibodies inhibiting the 5 ́→3 ́ polymerase activity and 3 ́→5 ́ exonuclease activity at room temperature, which enable Ultra Hi-Fi to perform greatly-specific Hot-Start PCRs. 2× Ultra Hi-Fi PCR MasterMix contains 2× Ultra Hi-Fi Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. Protective agents in this product are capable of repeated freeze-thaw cycles.

產品型號 規格 單價 庫存 購買數量
TTC-PE15 1ml -
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產品特點

 

長片段PCR實驗小秘訣請點我

  1. High Fidelity: The fidelity is 53-fold higher than Taq DNA polymerase

  2. Can amplify up to 40kb  

  3. Can tolerate GC rich template

  4. Can be used for direct PCR amplification

  5. Fast: 2kb/sec

實驗數據

Ultra Hi-Fi PCR MasterMix Fidelity Test: 

 

 

Ultra Hi-Fi Competition Test:

Enzyme Speed Test:

Ultra Hi-Fi Fragment Extension Test:

常見問題

Q1.為何PCR的enzyme通常cDNA放大的長度片段極限較短?

A:由於cDNA具有較複雜的二級結構,以及進行RT轉cDNA的時候並非是所有mRNA片段皆很規律的轉換,可能亦會是部分片段的轉cDNA,因此在做PCR放大的時候會具有較高的難度

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