TOOLSharp RNA Extractor

TOOLSharp RNA Extractor

TOOLSharp RNA Extractor is the most efficient reagent for isolation of total RNA from human, animal, and plant, bacterial and viral origin samples. This unique reagent provides higher yield and quality of isolated RNA than traditional reagents based on the single-step method. TOOLSharp RNA Extractor isolates pure and undegraded RNA that is ready for the following application without DNase treatment. TOOLSharp RNA Extractor separates RNA from other molecules in a single-step based on the interaction of phenol and guanidine with cellular components. No chloroform-induced phase separation is necessary to obtain pure RNA. A biological sample is homogenized or lysed in TOOLSharp RNA Extractor. DNA, proteins, polysaccharides and other molecules precipitate out of the homogenate/ lysate by addition of water and removing it by centrifugation. The pure RNA is isolated from the resulting supernatant by alcohol precipitation, followed by elution.

產品型號 規格 單價 庫存 購買數量
TTD-NRNA200 200ml -
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產品特點
  1. Fast: Obtain RNA in 1 hour

  2. No choloform: To remove DNA,protein,polysaccharides and other contaminants only with water.

  3. TOOLSharp RNA reagent isolate total RNA (including mRNA, rRNA, microRNA, etc.) in one tube.

  4. High RNA yield and purity.

  5. Suitable for any sample type. 

  6. Low sample amount limitation.

  7. Multiple application : RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.

實驗數據

TOOLSharp RNA Extraction quality test by 293T cells:

Q-Sep data of TOOLSharp RNA Extractor

常見問題

Q1.TOOLSharp RNA extractor當中有個BAN purification的(optional)步驟,請問這個可以做什麼功用?

A:此步驟適合用於DNA濃度高的樣本當中,有助於提升RNA的萃取純度

 

Q2.ShrapRNA extractor分層的原理?

A:SharpRNA原理同傳統的TRIzol方法,惟Buffer是有經過調整及優化的,因此分層原理是相同

 

Q3.SharpRNA extractor當中的BAN濃度要加多少?

A:使用純的BAN即可,每1ml加入5ul的BAN,濃度為0.5%

 

Q4.TOOLSharp RNA Extractor建議回溶的體積為多少?

A:一般RNA萃取建議回溶的體積為30-50ul,但實際的情況還是要依照萃取完的Pellet來決定

Q4.ShrarpRNA 是否能加入血液後做保存? 以及後續是否能用Qiagen RNeasy RNA mini kit抽RNA產物?

A:保存的問題基本上是不建議,但實際我們有測試過加入SharpRNA後放置於-80環境保存1-2週萃取仍沒有問題。 

另外後續要用Qiagen RNeasy RNA mini kit抽RNA的部分,基本上也是不建議的,但若要測試的話,可以在離心後取上清液的步驟將上青液加入Qiagen RNeasy RNA mini kit的Column接續做下去

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