DNA View

DNA View

  TOOLS DNA view is an innovated nucleic acid stain to replace highly toxic ethidium bromide (EtBr) for detecting nucleic acid agarose gels. It emits green fluorescence when bound to DNA and RNA. TOOLS DNA view is as sensitive as EtBr and the staining protocol is also very similar, however, compared to EtBr, which is known as a strong mutagen, TOOLS DNA view causes much fewer mutations in the Ames test. In addition, it has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. Most importantly, it is not considered hazardous waste, can be disposed of according to standard laboratories procedures, and is stable for years.

產品型號 規格 單價 庫存 購買數量
TT-DNA01 1ml - -
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產品特點
  1. Non-carcinogenic/allergic safe dye for DNA staining.

  2. Add TOOLS DNA view into agarose gel directly.

  3. The staining sensitivity is as high as Etbr.

  4. Cost effectively (1:20000)

 

Product Name
DNA View
Cat Number
TT-DNA01
Staining method
agarose gel + DNA View
Sample type
DNA
Dilution ratio
1:20000
Excitation
UV & blue light
Emission
537nm

 

實驗數據

參考文獻
  1. Fang, Cheng-Chung, et al. "The Small Molecule Inhibitor QLT-0267 Decreases the Production of Fibrin-Induced Inflammatory Cytokines and Prevents Post-Surgical Peritoneal Adhesions." Scientific reports 8.1 (2018): 9481.

  2. LIAO, Chen-Chieh, et al. First molecular identification of Vorticella sp. from freshwater shrimps in Tainan, Taiwan. International Journal for Parasitology: Parasites and Wildlife, 2018, 7.3: 415-422.

常見問題

Q1.DNA View是否可以用藍光做為激發光?

A:可以,藍光及UV 光都可以做為DNA view的激發光。

 

Q2.DNA view加入時是否需要等膠體降溫?

A:不需要,膠體降溫後反而會凝固影響DNA view混合的效果

 

Q3.DNA view建議濃度及製膠的厚度建議多少?

A:建議100ml加入2.5ul-5ul的DNA view

 

Q4.DNA view只能染DNA嗎?

A:不只,DNA view也是可以拿來染RNA的,基本就是用於染核酸

 

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