TOOLS Prestained Protein Marker

TOOLS Prestained Protein Marker

TOOLS Prestained Protein Marker is ready-to-use and pre-mixed with loading buffer for direct loading 5 μl on gel electrophoresis. This product does not need further dilution or addition of any reducing agent. After running the gel, the marker yields the following 10 discrete fragments (in KDa): 180, 140, 100, 75 (red), 60, 45, 35, 25(green), 15, and 10.

 

 

產品型號 規格 單價 庫存 購買數量
TM-PM10170 250ul -
TM-PM10170-10 10 x 250ul - -
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產品特點
  1. Size range : 10 proteins spanning 10 to 180 kDa 

  2. Ready-to-use : supplied in a loading buffer for direct loading on gels, no need to boil 

  3. Sharp bands : color-coded bands of similar intensity for easy visualization 

  4. Quality tested : each lot evaluated by SDS-PAGE and Western blotting 

  5. Membrane-compatible: colored bands transfer to membranes for Western blotting

實驗數據

Migration patterns of Prestained Protein Marker

in different electrophoretic conditions

 

 

Stability of Prestained Protein Marker after striping

參考文獻
  1. Lee CY, Chen SP, Su CH, Ho YC, Yang ML, Lee SS, Huang-Liu R, Yang CP, Chen CJ, Kuan YH. Zerumbone from Zingiber zerumbet Ameliorates Lipopolysaccharide-Induced ICAM-1 and Cytokines Expression via p38 MAPK/JNK-IκB/NF-κB Pathway in Mouse Model of Acute Lung Injury. Chin J Physiol. 2018 Jun;61(3):171-180. 

  2. Lee CY, Su CH, Tsai PK, Yang ML, Ho YC, Lee SS, Chen CH, Chen WY, Lin ML, Chen CJ, Chian CY, Huang-Liu R, Chang YL, Kuan YH. Cadmium nitrate-induced neuronal apoptosis is protected by N-acetyl-l-cysteine via reducing reactive oxygen species generation and mitochondria dysfunction. Biomed Pharmacother. 2018 Dec;108:448-456. 

  3. Chien KJ, Yang ML, Tsai PK, Su CH, Chen CH, Horng CT, Yeh CH, Chen WY, Lin ML, Chen CJ, Chian CY, Kuan YH. Safrole induced cytotoxicity, DNA damage, and apoptosis in macrophages via reactive oxygen species generation and Akt phosphorylation. Environ Toxicol Pharmacol. 2018 Dec;64:94-100. 

  4. Yang CC, Chang CK, Chang MT, Shyur LF. Plant galactolipid dLGG suppresses lung metastasis of melanoma through deregulating TNF-α-mediated pulmonary vascular permeability and circulating oxylipin dynamics in mice. Int J Cancer. 2018 Dec 15;143(12):3248-3261. doi: 10.1002/ijc.31663. Epub 2018 Sep 27. PMID: 29978476.

  5. Huang YH, Kuo HC, Yang YL, Wang FS. MicroRNA-29a is a key regulon that regulates BRD4 and mitigates liver fibrosis in mice by inhibiting hepatic stellate cell activation. Int J Med Sci. 2019 Jan 1;16(2):212-220. 

常見問題

Q1.為何10-170Protein marker再做WB的實驗會被抗體辨認到?

A:此情況是因為我們的Protein marker當中有一些序列是會被二抗辨認到,所以才會在做WB的時候會呈色

Q2. 為何Protein marker使用到最後的時候,會有掉色的情況產生?

A:若客戶每管Marker可以在2週內使用完畢的情況下,建議放在4C情況下保存,減少反覆凍溶的機率;若使用頻率低的情況,建議分裝出來使用。

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