HR Pre-Stained Protein Marker (10-170kDa)

HR Pre-Stained Protein Marker (10-170kDa)

HR Pre-Stain Protein Marker is ready-to-use and pre-mixed with loading buffer for direct loading 5 μl on gel electrophoresis. This product does not need further dilution or addition of any reducing agent. After running the gel, the marker yields the following 10 discrete fragments (in KDa): 170, 130, 93, 70 (red), 53, 41, 30, 22(green), 14, and 9.

 

 

產品型號 規格 單價 庫存 購買數量
TM-PM10170 250ul -
TM-PM10170-10 10 x 250ul - -
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產品特點
  1. Size range : 10 proteins spanning 9 to 170 kDa 

  2. Ready-to-use : supplied in a loading buffer for direct loading on gels, no need to boil 

  3. Sharp bands : color-coded bands of similar intensity for easy visualization 

  4. Quality tested : each lot evaluated by SDS-PAGE and Western blotting 

  5. Membrane-compatible: colored bands transfer to membranes for Western blotting

 

 

 

參考文獻
  1. LEE, Chien-Ying, et al. Zerumbone from Zingiber zerumbet Ameliorates Lipopolysaccharide-Induced ICAM-1 and Cytokines Expression via p38 MAPK/JNK-IκB/NF-κB Pathway in Mouse Model of Acute Lung Injury. Chin J Physiol, 2018, 61.3: 171-80.

  2. LEE, Chien-Ying, et al. Cadmium nitrate-induced neuronal apoptosis is protected by N-acetyl-l-cysteine via reducing reactive oxygen species generation and mitochondria dysfunction. Biomedicine & Pharmacotherapy, 2018, 108: 448-456.

  3. CHIEN, Kuang-Jen, et al. Safrole induced cytotoxicity, DNA damage, and apoptosis in macrophages via reactive oxygen species generation and Akt phosphorylation. Environmental toxicology and pharmacology, 2018, 64: 94-100.

  4. YANG, Chung‐Chih, et al. Plant galactolipid dLGG suppresses lung metastasis of melanoma through deregulating TNF‐α‐mediated pulmonary vascular permeability and circulating oxylipin dynamics in mice. International journal of cancer, 2018.

常見問題

Q1.為何10-170Protein marker再做WB的實驗會被抗體辨認到?

A:此情況是因為我們的Protein marker當中有一些序列是會被二抗辨認到,所以才會在做WB的時候會呈色

Q2. 為何Protein marker使用到最後的時候,會有掉色的情況產生?

A:若客戶每管Marker可以在2週內使用完畢的情況下,建議放在4C情況下保存,減少反覆凍溶的機率;若使用頻率低的情況,建議分裝出來使用。

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