HE Swift Cloning Kit
TOOLS HE Swift Cloning Kit is an advanced positive selection system for the highest efficiency cloning of PCR products generated with Pfu DNA polymerase, Taq DNA polymerase, or other thermo-stable DNA polymerases. Additionally, any other DNA fragment, either blunt or sticky-end, can be successfully cloned using the kit. Cloning is fast and efficient; ligation takes only 5 minutes and yields more than 99% positive clones.
The kit features the novel positive selection cloning vector pHE. This vector contains a lethal gene which is disrupted by ligation of a DNA insert into the cloning site. As a result, only cells with recombinant plasmids are able to propagate, eliminating the need for expensive blue/white screening.
The vector contains an expanded multiple cloning site, as well as a T7 promoter for in vitro transcription. Sequencing primers are included for convenient sequencing of the insert.
Quick and efficient: Blue/white screening is not necessary, and the whole ligation process can be finished within 5 min.
Sensitive and widely applied: Good for the ligation of low concentration fragments and long fragments, works for both blunt and sticky end product.
1. No need for blue white screening.
pHE Vector Map:
Fig 1. Insert product (size: 909 bp).
M, Marker; Lane 1, Insert PCR product.
Fig 2. Ligation mixture
M, Marker; Lane 1, ligation mixture
Fig 3. Colony PCR
- Wu, Tsung-Ru, et al. "Gut commensal Parabacteroides goldsteinii plays a predominant role in the anti-obesity effects of polysaccharides isolated from Hirsutella sinensis." Gut (2018): gutjnl-2017.