TOOLS 2xProbe qPCR Mix

TOOLS 2xProbe qPCR Mix

TOOLS 2xProbe qPCR Mix is optimized for real-time quantitative polymerase chain reaction (PCR) assays. This ready-to-load mix contains all components necessary to perform qPCR, except templates, primers, and probes. Probe designs vary but in its most common type, hydrolysis probe, a 5′ reporter fluorophore and a 3′ quencher are incorporated on a short oligonucleotide complementary to a target sequence. During each PCR cycle, the 5′ flap endonuclease domain of Taq DNA polymerase hydrolyzes the probe because a primer extends and the target sequence amplifies. This cleavage event causes separation of the reporter fluorophore from the quencher and results in an amplification-dependent increase in fluorescence. This master mix includes a chemically modified version of Taq polymerase with the hot-start capability to inhibit nonspecific amplification, simultaneously allowing high efficiency, sensitivity, and reproducibility in qPCR amplification. This product is suitable for high-speed multiplex qPCR analyses.

產品型號 規格 單價 庫存 購買數量
TTG-RKBA208 1 ml (100 rnx) -
  1. 簡單方便:可直接使用的mastermix

  2. 高效率與高穩定:本產品包括 化學修飾的Taq聚合酶,具有hot-start功能,可抑制非特異性的擴增

  3. 高專一性:本產品適用於 TaqMan®, Molecular Beacon 等高專一性qPCR系統

  4. 專門設計的ROX dye配方用以校正螢光背景訊號




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