TOOLS Epstein-Barr Virus (EBV) miRNA Multiplex RT-qPCR Assay
TOOLS EBV miRNA Multiplex RT-qPCR Assay includes designed and validated RT-qPCR primer/probe set targeting 44 respect EBV mature microRNAs (Table 1) that are suggested of crucial and potential roles in EBV related disease. This assay system utilizes a stem-looped primer to specifically extend the cDNA of target miRNA in reverse transcription (RT) and is an exclusively designed set for the probe-based qPCR assay to increase detection accuracy. Combined with TOOLSQuant II Fast RT kit and TOOLS Easy 2X probe qPCR mix, TOOLS EBV miRNA Multiplex RT-qPCR Assay System allows rapid, sensitive, and specific detection and quantification of 48 microRNAs in a total RNA sample respectively. TOOLS EBV miRNA Multiplex RT-qPCR Assay also includes the primer/probe sets for U6 and SNORD48 as the normalization controls and two exogenous miRNAs (cel-miR-39-3p, and cel-miR 238-3p) as spike-in controls
- 經過充分驗證：包含44個文獻支持、與引發癌症相關之EBV mature microRNAs
we using a serial dilution of synthetic cDNAs as templates in qPCR experiment to examined the #48 EBV primer/probe mix specificity. The synthetic cDNA templates (1X107 -1X101copies) were detected by qPCR reaction. The linear Range and sensitivity fell within the expected range.
Figure 1. High sensitivity with a range of synthetic cDNAs
We examined the #48 EBV primer Specificity using cross-hybridization of assay, using synthetic cDNAs as templates in qPCR experiment for assessing the corresponding qPCR primer/probe mix. Ct values (the threshold set as 0.2) are relevant to the Control Lot. The target signals fell within the expected range.The final assay conditions demonstrated no cross-hybridization of each primer.
Figure 2. Broad linear range and high sensitivity