TOOLS Epstein-Barr Virus (EBV) miRNA Multiplex RT-qPCR Assay

TOOLS Epstein-Barr Virus (EBV) miRNA Multiplex RT-qPCR Assay

TOOLS EBV miRNA Multiplex RT-qPCR Assay includes designed and validated RT-qPCR primer/probe set targeting 44 respect EBV mature microRNAs (Table 1) that are suggested of crucial and potential roles in EBV related disease. This assay system utilizes a stem-looped primer to specifically extend the cDNA of target miRNA in reverse transcription (RT) and is an exclusively designed set for the probe-based qPCR assay to increase detection accuracy. Combined with TOOLSQuant II Fast RT kit and TOOLS Easy 2X probe qPCR mix, TOOLS EBV miRNA Multiplex RT-qPCR Assay System allows rapid, sensitive, and specific detection and quantification of 48 microRNAs in a total RNA sample respectively. TOOLS EBV miRNA Multiplex RT-qPCR Assay also includes the primer/probe sets for U6 and SNORD48 as the normalization controls and two exogenous miRNAs (cel-miR-39-3p, and cel-miR 238-3p) as spike-in controls

產品型號 規格 單價 庫存 購買數量
TTH-EBV 10 RT rxn / 20 qPCR rxn -
加入購物車
產品特點
  1. 經過充分驗證:包含44個文獻支持、與引發癌症相關之EBV mature microRNAs
  2. 準確性高:結合反轉錄與幹環引物和專門針對成熟miRNA的TagMan探針。
  3. 簡單和快速
操作流程

實驗數據

Sensitivity Tests

we using a serial dilution of synthetic cDNAs as templates in qPCR experiment to examined the #48 EBV primer/probe mix specificity. The synthetic cDNA templates (1X107 -1X101copies) were detected by qPCR reaction. The linear Range and sensitivity fell within the expected range.

Figure 1. High sensitivity with a range of synthetic cDNAs

 

Specificity Tests

We examined the #48 EBV primer Specificity using cross-hybridization of assay, using synthetic cDNAs as templates in qPCR experiment for assessing the corresponding qPCR primer/probe mix. Ct values (the threshold set as 0.2) are relevant to the Control Lot. The target signals fell within the expected range.The final assay conditions demonstrated no cross-hybridization of each primer. 

Figure 2. Broad linear range and high sensitivity

訂閱圖爾思電子報

您可以從電子郵件中得到我們最新的消息與資訊

訂閱服務確認

已發送 Email 驗證信給你,請點擊信件連結以完成訂閱程序

訂閱失敗

暫時無法接受訂閱,請稍候重新嘗試