Taq and 2X PCR Master Mix

  • 2X SuperGreen PCR Master Mix

    2X SuperGreen PCR Master Mix

    2X SuperGreen PCR Master Mix is a 2× concentrated, optimized mixture composed of Taq DNA Polymerase, the NH4+ buffer system, dNTPs and MgCl2. An inert green dye and a stabilizer are also present to allow direct loading of the final products onto an agarose gel for analysis. The advantages of 2X SuperGreen PCR Master Mix include high convenience, sensitivity, specificity and stability. It minimizes man-made errors during PCR operating process. 2X SuperGreen PCR Master Mix is suitable for routine PCR reaction, amplification of complex templates such as AT rich templates and templates with secondary structure, and large-scale gene detection.

  • 2X SuperRed PCR Master Mix

    2X SuperRed PCR Master Mix

    2X SuperRed PCR Master Mix is a 2× concentrated, optimized mixture composed of Taq DNA Polymerase, the NH4+ buffer system, dNTPs and MgCl2. An inert red dye and a stabilizer are also present to allow direct loading of the final products onto an agarose gel for analysis. The advantages of 2X SuperRed PCR Master Mix include high convenience, sensitivity, specificity and stability. It minimizes man-made errors during PCR operating process. 2X SuperRed PCR Master Mix is suitable for routine PCR reaction, amplification of complex templates such as GC rich templates (>60%) and templates with secondary structure, and large-scale gene detection.

  • Tools Taq DNA Polymerase

    Tools Taq DNA Polymerase

    TOOLS Taq DNA Polymerase is a recombinant 94 kDa DNA polymerase expressed in an E.coli strain that carries the cloned Thermu aquaticus DNA Polymerase gene. It possesses both 5’-3’ polymerase and exonuclease activity, and has no detectable 3’-5’ exonuclease activity. The extension rate of TOOLS Taq DNA Polymerase is 1-2 kb/min in PCR. In addition, it has a 3’ adenylation activity. Thus, the PCR products can be used directly in TA-cloning procedures.

  • 2X Tools Taq PCR MasterMix with Loading Dye(Blue)

    2X Tools Taq PCR MasterMix with Loading Dye(Blue)

    2× ToolsTaq PCR MasterMix is a 2× concentrated, optimized mixture composed of ToolsTaq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The advantages of 2× ToolsTaq PCR MasterMix include high convenience, sensitivity, specificity and stability. It minimizes man-made errors during PCR operating process. 2× ToolsTaq PCR MasterMix is suitable for routine PCR reaction, amplification of complex templates such as GC-rich templates (>60%) and templates with secondary structure, and large-scale gene detection.

  • Tools Taq DNA Polymerase w/o Mg2+

    Tools Taq DNA Polymerase w/o Mg2+

    TOOLS Taq DNA Polymerase is a recombinant 94 kDa DNA polymerase expressed in an E.coli strain that carries the cloned Thermu aquaticus DNA Polymerase gene. It possesses both 5’-3’ polymerase and exonuclease activity, and has no detectable 3’-5’ exonuclease activity. The extension rate of TOOLS Taq DNA Polymerase is 1-2 kb/min in PCR. In addition, it has a 3’ adenylation activity. Thus, the PCR products can be used directly in TA-cloning procedures.

  • Extra Long Taq DNA Polymerase

    Extra Long Taq DNA Polymerase

    ExtraLong Taq DNA Polymerase, a high fidelity of polymerases with 3’-5’ exonuclease activity designed for amplifying large fragment. A wide range of PCR products can be generated, up to 40 kb from lambda DNA and 30 kb from human genomic DNA. It is also a better choice for amplifying complex template, such as GC-rich template. ExtraLong Taq Polymerase is suitable as a direct replacement for ordinary Taq Polymerase in most applications. PCR products produced with ExtraLong Taq Polymerase can be used in either TA cloning or blunt cloning.

  • Super Hi-Fi Taq DNA Polymerase

    Super Hi-Fi Taq DNA Polymerase

    Super Hi-Fi Tag DNA Polymerase is a special blend of Taq polymerase and Pfu Polymerase. It possesses both 5’-3’ polymerase and exonuclease activity. The advantages of Super Hi-Fi Tag DNA Polymerase are high productivity and fidelity. Comparing to regular Taq polymerase, Super Hi-Fi Tag can efficiently amplify large DNA fragments (20Kb for simple templates and 10Kb for complex templates). Besides, it possesses higher extension rate and amplification efficiency than Pfu DNA Polymerase. Super Hi-Fi Tag DNA Polymerase generates PCR products with 3’-dA overhangs that can be directly used in TA-cloning. To obtain higher cloning efficiency, however, PCR products could be purified and added 3-d'A overhangs before TA cloning procedures.

  • 2X Super Hi-Fi Taq PCR MasterMix with loading dye

    2X Super Hi-Fi Taq PCR MasterMix with loading dye

    2× Super Hi-Fi Tag PCR MasterMix is a 2× concentrated, optimized mixture composed of Super Hi-Fi Tag DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The advantages of 2×Super Hi-Fi Tag PCR MasterMix include high convenience, sensitivity, specificity and stability. It minimizes man-made errors during PCR operating process. 2×Super Hi-Fi Tag PCR MasterMix is suitable for routine PCR reaction, amplification of complex templates such as GC-rich templates (>60%) and templates with secondary structure.

  • 2x HotStart Taq PCR MasterMix with Loading Dye(blue)

    2x HotStart Taq PCR MasterMix with Loading Dye(blue)

    2× HotStart Taq PCR MasterMix is a 2× concentrated, optimized mixture composed of HotStart Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer.

  • 2x Ultra Hi-Fi PCR Master Mix

    2x Ultra Hi-Fi PCR Master Mix

    TOOLS 2× Ultra Hi-Fi PCR Master Mix is is a new generation superior enzyme based on Ultra Hi-Fi DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau-inhibiting factor newly added to Ultra Hi-Fi greatly improve its long-fragment amplification ability, specificity, and PCR yield. Ultra Hi-Fi is capable of amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of P Ultra Hi-Fi is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Ultra Hi-Fi has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. P Ultra Hi-Fi contains two monoclonal antibodies inhibiting the 5 ́→3 ́ polymerase activity and 3 ́→5 ́ exonuclease activity at room temperature, which enable Ultra Hi-Fi to perform greatly-specific Hot-Start PCRs. 2× Ultra Hi-Fi PCR MasterMix contains 2× Ultra Hi-Fi Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. Protective agents in this product are capable of repeated freeze-thaw cycles.

  • TOOLS Taq Plus Polymerase

    TOOLS Taq Plus Polymerase

    TOOLS Taq Plus Polymerase is a thermostable DNA polymerase that exhibits a 5 '→3 'polymerase activity and a 5 '→3 'exonuclease activity, with no 3 '→5 'exonuclease activity. Taq Plus Polymerase is purified from an Escherichia coli (E.coli) strain overexpressing the gene of Thermus aquaticus DNA Polymerase. No endonuclease, exonuclease, or bacterial DNA were detected in this kit. The PCR products contain A at the 3'-end and can be directly cloned into TA-Vectors.

  • 2x Ultra Taq PCR MasterMix

    2x Ultra Taq PCR MasterMix

    TOOLS 2× Ultra Taq PCR MasterMix contains Ultra-Taq DNA Polymerase, dNTP, and an optimized buffer system containing 3’→5’exonuclease activity. Its fidelity is 6 times greater than that of Taq DNA Polymerase. Compared with Taq DNA Polymerase, Ultra-Taq DNA DNA polymerase has stronger amplification performance, higher sensitivity, and is more tolerant of impurities within 5 kb amplifying range. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. It can amplify up to 10 kb from human genomic DNA or up to 15 kb from λ DNA. Protective agents in the TOOLS 2× Ultra Taq PCR MasterMix enable the resistance to repeated freeze-thaw cycles.

  • TOOLS 5x FineTaq Master Mix

    TOOLS 5x FineTaq Master Mix

    TOOLS 5x FineTaq Master Mix is ready-to-use PCR mixture for all PCR applications. It offers a convenient, reproducible PCR mix that minimizes the multiple handling steps associated with setting up a PCR. It is supplied as 5X concentrated ready-to-use mixture of Taq DNA polymerase, reaction buffer, MgCl2, dNTPs, and enzyme stabilizer. It can amplify up to 5kb specific DNA fragment from lambda DNA. The advantages of TOOLS 5x FineTaq Master Mix include high convenience, sensitivity, specificity and stability. It minimizes man-made errors during PCR operating process. TOOLS 5x FineTaq Master Mix is suitable for routine PCR reaction, amplification of complex templates and templates with secondary structure, and large-scale gene detection.

  • TOOLS Hotstart Polymerase

    TOOLS Hotstart Polymerase

    TOOLS Hotstart Polymerase a monoclonal antibody which can inhibit Taq DNA polymerase activity when bound to it. Due to its unique thermo stability and high affinity to Taq DNA polymerase, TOOLS Hotstart Polymerase Antibody blocks Taq DNA polymerase activity at temperatures up to 65°C but releases fully active polymerase after heat treatment at 95°C for 30 seconds. TOOLS Hotstart Polymerase DNA Polymerase is recombinant Taq DNA polymerase contained TOOLS Hotstart Polymerase antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 95°C, thereby providing an automatic “hot start” for Taq DNA polymerase in PCR, which provide increased sensitivity, specificity, and yield, while allowing assembly of reactions at room temperature. The use of this antibody helps reduce PCR optimization requirements, reaction set-up time and effort, handling of reaction components, and contamination risk. No modifications to PCR reactions or protocols are necessary. This product has a 5’→3’ DNA polymerase activity and a 5’→3’ exonuclease activity, and lacks a 3’→5’ proofreading function. The PCR products contain A at the 3'-end and can be directly cloned into TA-Vectors.

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