The CRISPR (clustered regularly interspaced short palindromic repeats) system is a prokaryotic adaptive immune system that consists of single guide RNA and Cas9 protein for performing genome editing in many organisms. sgRNA targets 23bp DNA sequence in length and ends with two guanines (GG) also called protospacer adjacent motif (PAM) resulting specific “cutting” of target sequence in the cell allows efficient genome engineering (knock-out / knock-in) in vitro or vivo.
TOOLS sgRNA mRNA (in-vitro transcribed) is a ready-to-use product containing a custom-designed sgRNA for excellent efficiency and specificity. Together with TOOLS Cas9 protein (Cat. No. TS-cas9-50) or eSpCas9 1.1 protein (Cat. No. TS-cas901-50), these ready-to-inject reagents are suitable for producing gene-editing animals via one-cell embryos injection. These products can also be used to directly cut the target sequence in vitro.