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  • TOOLS eSpCas9 (1.1) Protein

    TOOLS eSpCas9 (1.1) Protein

      The CRISPR (clustered regularly interspaced short palindromic repeats) system is a prokaryotic adaptive immune system that consists of single guide RNA and Cas9 protein for performing genome editing in many organisms. 

    TOOLS eSpCas9 (1.1) protein (K848A/K1003A/R1060A) is a purified nuclease from E coli using Cas9 gene of Streptococcus pyogenes. It is a ready-to-use product containing a nuclear localization signal (NLS) for genome editing experiments resulting site-specific cleavage of double-stranded DNA.

  • TOOLS wtCas9 Protein

    TOOLS wtCas9 Protein

    The CRISPR (clustered regularly interspaced short palindromic repeats) system is a prokaryotic adaptive immune system that consists of single guide RNA and Cas9 protein for performing genome editing in many organisms. 

    TOOLS Cas9 protein is a purified nuclease from E. coli using Cas9 gene of Streptococcus pyogenes. It is a ready-to-use product containing a nuclear localization signal (NLS) for genome editing experiments resulting site-specific cleavage of double-stranded DNA.

  • TOOLS AsCpf1 Protein

    TOOLS AsCpf1 Protein

    The CRISPR (clustered regularly interspaced short palindromic repeats) system is a prokaryotic adaptive immune system that consists of single guide RNA and Cas9 protein for performing genome editing in many organisms. CRISPR/Cpf1 (Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella 1) is an RNA-guided endonuclease of a class II CRISPR/Cas system. This acquired immune mechanism is found in Prevotella and Francisella bacteria. It prevents genetic damage from viruses. Cpf1 genes are associated with the CRISPR locus, coding for a smaller and simpler endonuclease than Cas9 and it also uses a guide RNA to find and cleave viral DNA.

    TOOLS AsCpf1 protein is a purified nuclease from E coli using AsCpf1 gene of Acidaminococcus. It is a ready-to-use product containing a nuclear localization signal (NLS) for genome editing experiments resulting site-specific cleavage of double stranded DNA.

  • Custom Pre-Made CRISPR/Cas System (for cell line)

    Custom Pre-Made CRISPR/Cas System (for cell line)

       CRISPR (clusters of regularly interspaced palindromic repeats)/Cas (CRISPR-associated) system is derived from an adaptive immune response against invading phages and plasmids in bacteria and archaea termed type II CRISPR. This genome editing technology is now widely used for making gene knockout/knock-in animals and plants as well as genome-modified cell lines. 

      TOOLS provides a custom CRIPSR/Cas system in which ready-to-use system for your gene/locus will be developed upon request. Our special CRISPR/Cas System has two separated plasmids, one is sgRNA over-expressing plasmid and the other is Cas9 protein over-expressing plasmid. So, you can adjust ratio of these two plasmids to reach the best effiency during transfecion. Our sgRNA are modified to increase the binding ability with target DNA. 

  • Custom Pre-Made CRISPR/Cas9 System (for animal model)

    Custom Pre-Made CRISPR/Cas9 System (for animal model)

    CRISPR (clusters of regularly interspaced palindromic repeats)/Cas (CRISPR-associated) system is derived from an adaptive immune response against invading phages and plasmids in bacteria and archaea termed type II CRISPR. This genome editing technology is now widely used for making gene knockout/knock-in animals and plants as well as genome-modified cell lines. 

    TOOLS Custom Pre-Made CRISPR/Cas System are ready-to-miroinjection system for your genome engineering experiments. Our CRISPR/Cas systems are composed of a sgRNA mRNA that has been custom-designed for your target gene and recombinant Cas9 protein with minimal toxicity in animal embryos.

    TOOLS Cas9 protein is a purified nuclease from E. coli using Cas9 gene of Streptococcus pyogenes. It is a ready-to-use product containing a nuclear localization signal (NLS) for genome editing experiments resulting site-specific cleavage of double-stranded DNA.

    Quotations and Ordering : 

    For quotation requests and questions, you may contact us by phone, email, fax, or via our online quotation system.

  • EasyPrep HY Genomic DNA Extraction Kit

    EasyPrep HY Genomic DNA Extraction Kit

    EasyPrep HY Genomic DNA Extraction Kit is based on special buffer system for many kinds of sample’s gDNA extraction. Simple centrifugation processing completely removes contaminants and enzyme inhibitors such as proteins and divalent cations. Purified DNA is rehydrated by DNA Dissolving Buffer, ready for use in downstream applications.
    DNA purified by EasyPrep HY Genomic DNA Extraction Kit is highly suitable for restriction analysis, PCR analysis, Southern blotting, and cDNA library.

  • EasyPrep Genomic DNA Extraction Kit

    EasyPrep Genomic DNA Extraction Kit

    EasyPrep Genomic DNA Kit is based on silica membrane technology and special buffer system for many kinds of sample’s gDNA extraction. The spin column made of new type silica membrane can bind DNA optimally on given salt and pH conditions. Simple centrifugation processing completely removes contaminants and enzyme inhibitors such as proteins and divalent cations. Purified DNA is eluted in low-salt buffer or water, ready for use in downstream applications. DNA purified by EasyPrep Genomic DNA Kit is highly suited for restriction analysis, PCR analysis, Southern blotting, and cDNA library.

  • EasyPrep Stool Genomic DNA Kit

    EasyPrep Stool Genomic DNA Kit

    EasyPrep Stool Genomic DNA Kit is based on silica membrane technology and special buffer system with RepressIn Tablet for stool sample gDNA extraction. The spin column made of new type silica membrane can bind DNA optimally on given salt and pH conditions. Simple centrifugation processing completely removes impurity, proteins and other organic compound. Special RepressIn Tablet can absorb impurity from sample easily. The gDNA isolated with these products is of high quality, high purity and full-length. Purified gDNA is ready for use in downstream applications such as PCR.

  • EasyPrep Total RNA Kit

    EasyPrep Total RNA Kit

    EasyPrep Total RNA Kit provides a new RNA isolation technology based on Guanidine Thiocyanate/ Phenyl method. It contains a unique buffer RBZ that minimizes the contamination of genomic DNA and protein. This Kit efficiently isolates high pure RNA from blood, cells, tissues and plant samples in one hour. The purified RNA is ready-to-use in downstream applications such as: RT-PCR and real-time RT-PCR, gene-chips assay, northern blot, poly A screening, in vitro transcript, and molecular cloning.

  • TOOLSmart RNA Extractor

    TOOLSmart RNA Extractor

     TOOLSmart RNA Extractor is developed based on regular nucleic acid extraction Reagent with the addition of indicator. It has better lysis ability and higher sensitivity. It could be used to isolate total RNA from samples like virus, bacteria, plant tissue and body fluid. It maintains the integrity of RNA while disrupting cells and dissolving cell components during sample homogenization. TOOLSmart RNA Extractor can be applied to isolate RNA from both small amount of samples (50-100 mg tissue or 5 × 106cells) and large amount of samples (≥1 g tissue or ≥107cells) of human, animal, plant or bacteria, within one hour. DNA and protein contamination is eliminated thoroughly from RNA products. Isolated RNA can be used in Northern Blot, Dot Blot, poly(A) selection, in vitro translation, RNase protection assay and molecular cloning.

  • EasyPrep Blood RNA Purification Kit

    EasyPrep Blood RNA Purification Kit

    EasyPrep Blood RNAprep Purification Kit provides a fast, simple, and cost-effective method for purification of total RNA from blood. The purified RNA is ready for use in downstream applications such as RT-PCR and real-time RT-PCR, micro array, northern blot, dot blot, polyA screening, in vitro transcription, and molecular cloning.

  • TOOLSharp RNA Extractor

    TOOLSharp RNA Extractor

    TOOLSharp RNA Extractor is the most efficient reagent for isolation of total RNA from human, animal, and plant, bacterial and viral origin samples. This unique reagent provides higher yield and quality of isolated RNA than traditional reagents based on the single-step method. TOOLSharp RNA Extractor isolates pure and undegraded RNA that is ready for the following application without DNase treatment. TOOLSharp RNA Extractor separates RNA from other molecules in a single-step based on the interaction of phenol and guanidine with cellular components. No chloroform-induced phase separation is necessary to obtain pure RNA. A biological sample is homogenized or lysed in TOOLSharp RNA Extractor. DNA, proteins, polysaccharides and other molecules precipitate out of the homogenate/ lysate by addition of water and removing it by centrifugation. The pure RNA is isolated from the resulting supernatant by alcohol precipitation, followed by elution.

  • TOOLS Plasmid Mini kit

    TOOLS Plasmid Mini kit

    TOOLS Plasmid Mini kit is based on alkaline lysis technology followed by adsorption of DNA onto silica membrane in the presence of high salt. Plasmid DNA purified with this product is immediately ready for use. Phenol extraction and ethanol precipitation are not required. High-quality plasmid DNA is eluted in a small volume of Tris Buffer or deionized water. This protocol is designed for purification of up to 30 μg of plasmid DNA (<12kb) from 1-4 ml overnight cultures of E. coli in LB (Luria-Bertani) medium. Plasmid DNA prepared by this kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in vitro translation, and transfection of robust cells.

  • EasyPrep High Pure Plasmid Mini Plus Extraction kit

    EasyPrep High Pure Plasmid Mini Plus Extraction kit

    EasyPrep High Pure Plasmid Mini Plus Extraction kit is based on alkaline lysis technology followed by adsorption of DNA onto silica membrane in the presence of high salt. Plasmid DNA purified with this product is immediately ready for use. Phenol extraction and ethanol precipitation are not required. High-quality plasmid DNA is eluted in a small volume of Tris Buffer or deionized water. This protocol is designed for purification of up to 70 μg of plasmid DNA from 5-15 ml overnight culture of E. coli in LB (Luria-Bertani) medium. Plasmid DNA prepared by EasyPrep High Pure Plasmid Mini plus kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in vitro translation, and transfection of robust cells.

  • EasyPrep HY-Midi Plasmid Extraction Kit

    EasyPrep HY-Midi Plasmid Extraction Kit

    EasyPrep HY-Midi Plasmid Extraction Kit adopts specially designed buffer system that is used in conjunction with isopropanol precipitation to achieve fast and efficient purification of high pure plasmid DNA. Plasmid DNA prepared by this Kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in vitro translation, and transfection of cell lines.
    Recommended bacterial culture volume: 500-1500 ug plasmid with 100 ml bacterial culture for high-copy vectors; and 200-400 ug plasmid with 200 ml bacterial culture for low- copy vectors.

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