TOOLSite-Directed Mutagenesis Kit

  • TOOLSite-Directed Mutagenesis Kit

    TOOLSite-Directed Mutagenesis Kit

    TOOLSite-Directed Mutagenesis Kit is designed for rapid site-directed mutagenesis based on new rapid cloning technology. The amplified target fragments are digested by DpnI, cyclized by Toolsitase/buffer, and then transformed to E. coli to finish the site-directed mutagenesis. The kit consists of two modules: Hi-Fi DNA Polymerase amplification module and rapid cloning module. The ultra-fidelity of Hi-Fi DNA Polymerase can significantly reduce the possibility of unexpected mutations during amplification of up to 20 kb in length. The rapid cloning module allows homologous recombination reactions to replace the conventional annealing ring-forming reactions, requiring much less template and more flexible primer design strategy. Due to the efficient seamless splicing of the two PCR products by Toolsitase technology, the kit can complete the two separate sites mutation by single amplification reaction. DpnI digested products of the specific amplicons can be directly added into recombination reaction without purification. Highly optimized reaction buffer, fast operating procedures and amazing site-directed mutagenesis efficiency make TOOLSite-Directed Mutagenesis Kit the preferred kit for DNA site mutation.

  • TOOLSite-Directed Multi-Mutagenesis Kit

    TOOLSite-Directed Multi-Mutagenesis Kit

    TOOLSite-Directed Multi-Mutagenesis Kit is designed for rapid site-directed mutagenesis based on new rapid cloning technology. This kit can fulfill 3-5 separate site-directed mutagenesis in one reaction.
    The amplified target fragments are digested by DpnI, cyclized by Multi-Toolsitase/buffer, and then transformed to E. coli to finish the site-directed mutagenesis. The kit consists of two modules: Hi-Fi DNA Polymerase amplification module and rapid cloning module. The ultra-fidelity of Hi-Fi DNA Polymerase can significantly reduce the possibility of unexpected mutations during amplification of up to 20 kb in length. The rapid cloning module allows homologous recombination reactions to replace the conventional annealing ring-forming reactions, requiring much less template and more flexible primer design strategy. The kit is equipped with Multi-Toolsitase, a recombinant enzyme specifically optimized for multi-sites-directed mutations. DpnI digestion of the specific amplification products can be directly applied in recombination reaction without purification. Highly optimized reaction buffer, fast operating procedures and amazing site-directed mutagenesis efficiency make TOOLSite-Directed Mutagenesis Kit the preferred kit for DNA multi-sites mutation.

訂閱圖爾思電子報

您可以從電子郵件中得到我們最新的消息與資訊

訂閱服務確認

已發送 Email 驗證信給你,請點擊信件連結以完成訂閱程序

訂閱失敗

暫時無法接受訂閱,請稍候重新嘗試