TOOLS Easy 2×Probe qPCR Mix is suitable for real-time quantitative polymerase chain reaction (PCR) assays. This ready-to-load mix contains dNTP/dUTP mix, Mg2+, DNA polymerase, dUTP/UDG anticontamination system, specific ROX Reference Dye, and all components necessary to perform qPCR, except templates, primers, and probes. The polymerase in this kit is a chemically modified hot-start DNA polymerase. The kit contains optimized buffer that effectively inhibits nonspecific amplification and significantly improves the efficiency of qPCR reactions. The dUTP/UDG anticontamination system added to the mix eliminates the product contamination of qPCR reactions.
The 2× reaction mix can be directly used for a robust and low-template qPCR with high sensitivity, specificity, and reliability. TOOLS Easy 2×Probe qPCR Mix also contains ROX Reference Dye suitable for all qPCR instruments, and no adjustments are required for the dye concentration in different instruments.
TOOLS One-Step Probe RT-qPCR Mix is specially designed to perform rapid real-time PCR using probe method (TaqMan®, Molecular Beacon, etc). The kit allows both reverse transcription and gene amplification to take place in a single tube, which avoids cross contamination between samples and improves the sensitivity of detection.
The 25×TOOLS Enzyme Mix contains a highly efficient reverse transcriptase expressed by engineering bacteria; a further-modified hot start Taq DNA polymerase, which provides high efficiency and accuracy for the amplify reaction; and RNase inhibitor. With a special modified hydrophobic motif, this RTase exerts a significant affinity for RNA and facilitates transcription through of RNA templates, especially for templates with high GC content or complex secondary structures. This product contains appropriate ion concentration, dNTPs and PCR enhancer. It stabilizes polymerases and maintain their efficiency within whole reaction process.