TOOLS One-Step Probe RT-qPCR Mix is specially designed to perform rapid real-time PCR using probe method (TaqMan®, Molecular Beacon, etc). The kit allows both reverse transcription and gene amplification to take place in a single tube, which avoids cross contamination between samples and improves the sensitivity of detection.
The 25×TOOLS Enzyme Mix contains a highly efficient reverse transcriptase expressed by engineering bacteria; a further-modified hot start Taq DNA polymerase, which provides high efficiency and accuracy for the amplify reaction; and RNase inhibitor. With a special modified hydrophobic motif, this RTase exerts a significant affinity for RNA and facilitates transcription through of RNA templates, especially for templates with high GC content or complex secondary structures. This product contains appropriate ion concentration, dNTPs and PCR enhancer. It stabilizes polymerases and maintain their efficiency within whole reaction process.
TOOLS 2X Probe qPCR Mix is optimized for real-time quantitative polymerase chain reaction (PCR) assays. This ready-to-load mix contains all components necessary to perform qPCR, except templates, primers, and probes. Probe designs vary but in its most common type, hydrolysis probe, a 5′ reporter fluorophore and a 3′ quencher are incorporated on a short oligonucleotide complementary to a target sequence. During each PCR cycle, the 5′ flap endonuclease domain of Taq DNA polymerase hydrolyzes the probe because a primer extends and the target sequence amplifies. This cleavage event causes separation of the reporter fluorophore from the quencher and results in an amplification-dependent increase in fluorescence. This master mix includes a chemically modified version of Taq polymerase with hot-start capability to inhibit nonspecific amplification, simultaneously allowing high efficiency, sensitivity, and
reproducibility in qPCR amplification. This product is suitable for high-speed multiplex qPCR analyses.