Taq DNA Polymerases

  • Tools Taq DNA Polymerase

    Tools Taq DNA Polymerase

    TOOLS Taq DNA Polymerase is a recombinant 94 kDa DNA polymerase expressed in an E.coli strain that carries the cloned Thermu aquaticus DNA Polymerase gene. It possesses both 5’-3’ polymerase and exonuclease activity, and has no detectable 3’-5’ exonuclease activity. The extension rate of TOOLS Taq DNA Polymerase is 1-2 kb/min in PCR. In addition, it has a 3’ adenylation activity. Thus, the PCR products can be used directly in TA-cloning procedures.

  • Tools Taq DNA Polymerase w/o Mg2+

    Tools Taq DNA Polymerase w/o Mg2+

    TOOLS Taq DNA Polymerase is a recombinant 94 kDa DNA polymerase expressed in an E.coli strain that carries the cloned Thermu aquaticus DNA Polymerase gene. It possesses both 5’-3’ polymerase and exonuclease activity, and has no detectable 3’-5’ exonuclease activity. The extension rate of TOOLS Taq DNA Polymerase is 1-2 kb/min in PCR. In addition, it has a 3’ adenylation activity. Thus, the PCR products can be used directly in TA-cloning procedures.

  • Extra Long Taq DNA Polymerase

    Extra Long Taq DNA Polymerase

    ExtraLong Taq DNA Polymerase, a high fidelity of polymerases with 3’-5’ exonuclease activity designed for amplifying large fragment. A wide range of PCR products can be generated, up to 40 kb from lambda DNA and 30 kb from human genomic DNA. It is also a better choice for amplifying complex template, such as GC-rich template. ExtraLong Taq Polymerase is suitable as a direct replacement for ordinary Taq Polymerase in most applications. PCR products produced with ExtraLong Taq Polymerase can be used in either TA cloning or blunt cloning.

  • Super Hi-Fi Taq DNA Polymerase

    Super Hi-Fi Taq DNA Polymerase

    Super Hi-Fi Tag DNA Polymerase is a special blend of Taq polymerase and Pfu Polymerase. It possesses both 5’-3’ polymerase and exonuclease activity. The advantages of Super Hi-Fi Tag DNA Polymerase are high productivity and fidelity. Comparing to regular Taq polymerase, Super Hi-Fi Tag can efficiently amplify large DNA fragments (20Kb for simple templates and 10Kb for complex templates). Besides, it possesses higher extension rate and amplification efficiency than Pfu DNA Polymerase. Super Hi-Fi Tag DNA Polymerase generates PCR products with 3’-dA overhangs that can be directly used in TA-cloning. To obtain higher cloning efficiency, however, PCR products could be purified and added 3-d'A overhangs before TA cloning procedures.

  • TOOLS Taq Plus Polymerase

    TOOLS Taq Plus Polymerase

    TOOLS Taq Plus Polymerase is a thermostable DNA polymerase that exhibits a 5 '→3 'polymerase activity and a 5 '→3 'exonuclease activity, with no 3 '→5 'exonuclease activity. Taq Plus Polymerase is purified from an Escherichia coli (E.coli) strain overexpressing the gene of Thermus aquaticus DNA Polymerase. No endonuclease, exonuclease, or bacterial DNA were detected in this kit. The PCR products contain A at the 3'-end and can be directly cloned into TA-Vectors.

  • TOOLS Hotstart Polymerase

    TOOLS Hotstart Polymerase

    TOOLS Hotstart Polymerase a monoclonal antibody which can inhibit Taq DNA polymerase activity when bound to it. Due to its unique thermo stability and high affinity to Taq DNA polymerase, TOOLS Hotstart Polymerase Antibody blocks Taq DNA polymerase activity at temperatures up to 65°C but releases fully active polymerase after heat treatment at 95°C for 30 seconds. TOOLS Hotstart Polymerase DNA Polymerase is recombinant Taq DNA polymerase contained TOOLS Hotstart Polymerase antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 95°C, thereby providing an automatic “hot start” for Taq DNA polymerase in PCR, which provide increased sensitivity, specificity, and yield, while allowing assembly of reactions at room temperature. The use of this antibody helps reduce PCR optimization requirements, reaction set-up time and effort, handling of reaction components, and contamination risk. No modifications to PCR reactions or protocols are necessary. This product has a 5’→3’ DNA polymerase activity and a 5’→3’ exonuclease activity, and lacks a 3’→5’ proofreading function. The PCR products contain A at the 3'-end and can be directly cloned into TA-Vectors.

訂閱電子報