2X PCR Master Mix w/o Loading Dye

  • 2x Ultra Hi-Fi PCR Master Mix

    2x Ultra Hi-Fi PCR Master Mix

    TOOLS 2× Ultra Hi-Fi PCR Master Mix is is a new generation superior enzyme based on Ultra Hi-Fi DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau-inhibiting factor newly added to Ultra Hi-Fi greatly improve its long-fragment amplification ability, specificity, and PCR yield. Ultra Hi-Fi is capable of amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of P Ultra Hi-Fi is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Ultra Hi-Fi has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. P Ultra Hi-Fi contains two monoclonal antibodies inhibiting the 5 ́→3 ́ polymerase activity and 3 ́→5 ́ exonuclease activity at room temperature, which enable Ultra Hi-Fi to perform greatly-specific Hot-Start PCRs. 2× Ultra Hi-Fi PCR MasterMix contains 2× Ultra Hi-Fi Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. Protective agents in this product are capable of repeated freeze-thaw cycles.

  • 2x Ultra Taq PCR MasterMix

    2x Ultra Taq PCR MasterMix

    TOOLS 2× Ultra Taq PCR MasterMix contains Ultra-Taq DNA Polymerase, dNTP, and an optimized buffer system containing 3’→5’exonuclease activity. Its fidelity is 6 times greater than that of Taq DNA Polymerase. Compared with Taq DNA Polymerase, Ultra-Taq DNA DNA polymerase has stronger amplification performance, higher sensitivity, and is more tolerant of impurities within 5 kb amplifying range. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. It can amplify up to 10 kb from human genomic DNA or up to 15 kb from λ DNA. Protective agents in the TOOLS 2× Ultra Taq PCR MasterMix enable the resistance to repeated freeze-thaw cycles.

  • TOOLS 5x FineTaq Master Mix

    TOOLS 5x FineTaq Master Mix

    TOOLS 5x FineTaq Master Mix is ready-to-use PCR mixture for all PCR applications. It offers a convenient, reproducible PCR mix that minimizes the multiple handling steps associated with setting up a PCR. It is supplied as 5X concentrated ready-to-use mixture of Taq DNA polymerase, reaction buffer, MgCl2, dNTPs, and enzyme stabilizer. It can amplify up to 5kb specific DNA fragment from lambda DNA. The advantages of TOOLS 5x FineTaq Master Mix include high convenience, sensitivity, specificity and stability. It minimizes man-made errors during PCR operating process. TOOLS 5x FineTaq Master Mix is suitable for routine PCR reaction, amplification of complex templates and templates with secondary structure, and large-scale gene detection.

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