TOOLS 2× Ultra Hi-Fi Taq PCR MasterMix is is a new generation superior enzyme based on Ultra Hi-Fi DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau-inhibiting factor newly added to Ultra Hi-Fi greatly improve its long-fragment amplification ability, specificity, and PCR yield. Ultra Hi-Fi is capable of amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of Ultra Hi-Fi is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Ultra Hi-Fi has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Ultra Hi-Fi contains two monoclonal antibodies inhibiting the 5 ́→3 ́ polymerase activity and 3 ́→5 ́ exonuclease activity at room temperature, which enable Ultra Hi-Fi to perform greatly-specific Hot-Start PCRs. 2× Ultra Hi-Fi Taq PCR MasterMix contains 2× Ultra Hi-Fi Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. Protective agents this product enable the resistance to repeated freeze-thaw cycles.