Cell Assay Kit

  • TOOLS Cell Counting (CCK-8) kit

    TOOLS Cell Counting (CCK-8) kit

    TOOLS Cell Counting (CCK-8) kit is a ready-to-use solution without premixing of components. Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give a yellow colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.

  • MTT Cell Proliferation and Cytotoxicity Assay Kit

    MTT Cell Proliferation and Cytotoxicity Assay Kit

    MTT Cell Proliferation Assay Kit provides a simple method for determination of cell number using standard microplate absorbance readers. Determination of cell growth rates is widely used in the testing of drug action, cytotoxic agents and screening other biologically active compounds. Several methods can be used for such determinations, but indirect approaches using fluorescent or chromogenic indicators provide the most rapid and large-scale assays.Among such procedures, the MTT assay developed by Mossman1 is still among one of the most versatile and popular assays. The MTT assay involves the conversion of the water-soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to an insoluble formazan.2-4 The formazan is then solubilized, and the concentration determined by optical density at 570 nm. The result is a sensitive assay with excellent linearity up to approximately 96 cells per well.

  • Telomere Length qPCR assay kit

    Telomere Length qPCR assay kit

    Telomeres are nucleoprotein structures that cap the ends of chromosomes. The integrity of the telomere structure and its DNA (TTAGGG)n repeat sequence is critical for the protection of the ends of chromosomes from degradation and in maintaining overall genomic stability. Telomere length, therefore, decreases over time and may predict lifespan. Telomere shortening has negative effects on health conditions and has been linked to many health issues including aging and cancer.

    Telomere Length qPCR assay kit is designed to directly measure the average telomere length of a human cell population. The telomere primer mix recognizes and amplifies telomere sequences. The single copy gene (SCG) primer mix recognizes specific target gene as a control for amplification for every sample performed and to determine genome copies per sample, and serves as reference for data normalization. The Telomere standard DNA sample with known telomere length serves as a reference for calculating the telomere length of target samples.

  • TOOLS Mycoplasma Detection Kit

    TOOLS Mycoplasma Detection Kit

    TOOLS Mycoplasma Detection Kit allows for quick and reliable screening of cell cultures for mycoplasma contamination. Highly undesirable, easily acquired and notorious for an elusive onset of infection, mycoplasmas tend to alter the infected cells at a molecular level, ultimately leading to visible changes in cell morphology and growth characteristics. Timely detection of mycoplasmas in cell cultures is desirable in order to deter wide-spread contamination and save on the costly efforts of elimination. Designed to perform with high specificity and sensitivity, this Kit helps minimize false positives while ensuring coverage over 200 species/strains of mycoplasmas with a quick protocol for routine screening.

  • TOOLShinning Dual Luciferase Reporter Assay Kit

    TOOLShinning Dual Luciferase Reporter Assay Kit

    TOOLShinning Dual Luciferase Reporter Assay Kit detects gene regulation by transfecting cells with a reporter plasmid and measuring the fluorescence intensity of Luciferin substrate to reflect the level of Luciferase expression. To achieve dual luciferase reporter gene detection, firefly luciferase is detected with Luciferin as a substrate, and renilla luciferase is detected with coelenterazine as a substrate, while inhibiting the activity of Firefly luciferase. Dual Luciferase Reporter Assay Kit can detect the expression of Luciferase regulated by gene elements sensitively and efficiently. Usually, the transcriptional regulatory element is cloned upstream of Firefly luciferase, or the 3'-UTR regulatory region is cloned downstream of firefly luciferase. The transfected cells are induced by corresponding stimulator and lysed to determine the luciferase activity. The stimulatory-inducing effect of the regulatory elements is evaluated by luciferase activity. Renilla luciferase acts as an internal reference for correcting transfection efficiency to eliminate differences in cell number and transfection efficiency between wells. Firefly luciferase catalyzes the emission of Luciferin at 560 nm, and renilla luciferase catalyzes the emission of coelenterazine at 465 nm.




已發送 Email 驗證信給你,請點擊信件連結以完成訂閱程序